Standardized Southern Blot Workshop Technique

Marcadet, A.; O’Connell, P.; Cohen, David

doi:10.1007/978-1-4612-3552-1_116

Cited by 59 publications

(37 citation statements)

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“…Air-dried DNA was resuspended in TRIS EDTA and stored at −80°C before use at a concentration of 1 μg/μl [9,10]. It is noteworthy that this method did not use glass microbeads to increase the efficiency of bacterial DNA extraction, as commonly done in current methods.…”

Section: Study Overviewmentioning

confidence: 99%

Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept

Amar

Sérino²,

Lange³

et al. 2011

Diabetologia

285

213

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Aims/hypothesis Evidence suggests that bacterial components in blood could play an early role in events leading to diabetes. To test this hypothesis, we studied the capacity of a broadly specific bacterial marker (16S rDNA) to predict the onset of diabetes and obesity in a general population. Methods Data from an Epidemiological Study on the Insulin Resistance Syndrome (D.E.S.I.R.) is a longitudinal J. Amar and M. Serino contributed equally to this study. Electronic supplementary material The online version of this article

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Section: Study Overviewmentioning

confidence: 99%

Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept

Amar

Sérino²,

Lange³

et al. 2011

Diabetologia

285

213

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“…20 Insertion/deletion polymorphism was analyzed after amplification of the corresponding region by the polymerase chain reaction (PCR) as described by Visvikis et al 5 The primers for PCR were obtained from Eurogentec SA (Belgium). The product gives rise to a 93-bp fragment corresponding to the insertion (Ins) allele or an 84-bp fragment corresponding to the deletion (Del) allele.…”

Section: Dna Analysismentioning

confidence: 99%

Apolipoprotein B signal peptide polymorphism and plasma LDL-cholesterol response to low-calorie diet

Jemaa

Mebazaa

Fumeron³

2004

Int J Obes

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“…14 Genomic DNA from included subjects were extracted by standard techniques. 15 Identification of polymorphisms Search for new di-allelic polymorphisms by PCR/SSCP or REF From the known genomic structure of the human AGT gene 16 several overlapping sets of oligonucleotides (sequences available upon request) were designed to perform a systematic search for new polymorphisms by Polymerase-Chain Reaction/Single-Strand Conformation Analysis (PCR/SSCA) 17,18 on a 1155-bp region containing exon 5 and a part of the 3'-flanking region with two downstream enhancer core elements (+1399 to +1478 and +2191 to +2214). 19,20 Restriction endonuclease fingerprinting (REF) 21 was performed as a modification of the SSCA method to detect genetic variants in a 395-bp fragment (Table 2) containing the 24-bp enhancer core element.…”

Section: Family Datamentioning

confidence: 99%

Detection of putative functional angiotensinogen (AGT) gene variants controlling plasma AGT levels by combined segregation-linkage analysis

Brand

Chatelain²,

Paillard

et al. 2002

Eur J Hum Genet

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Previous studies have suggested that angiotensinogen (AGT) gene variants are associated with increased plasma AGT levels, and may also contribute towards the inherited component of predisposition to essential hypertension in humans. To explore the potential functionality of several AGT polymorphisms and estimate their effects, together with other sources of familial correlations, on plasma AGT, we undertook a large study involving 545 healthy French volunteers in 130 nuclear families that include 285 offspring. Plasma AGT levels were measured in all participants, and bi-allelic AGT variants were analysed as candidate functional variants at three sites in the 5'-flanking region (C-532T, A-20C, G-6A), two sites in exon 2 (M235T, T174M) and two newly identified variant sites in the untranslated sequence of exon 5 and the 3'-flanking region (C+2054A, C+2127T) of the gene. Analysis with the class D regressive model showed significant effects influencing plasma AGT levels of all AGT polymorphisms tested, with the exception of T174M. The most significant result was found at C-532T (P=0.000001), which accounts for 4.3% of total plasma AGT variability in parents and 5.5% in offspring, with substantial residual familial correlations. Maximum likelihood estimates of haplotype frequencies and tests of linkage disequilibrium between each AGT polymorphism and a putative QTL are in agreement with a complete confounding of C-532T with the QTL, when taking into account sex and generation specific effects of the QTL. However, further combined segregation-linkage analyses showed significant evidence for additional effects of G-6A, M235T and C+2054A polymorphisms after accounting for C-532T, which supports a complex model with at least two functional variants within the AGT gene controlling AGT levels.

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Standardized Southern Blot Workshop Technique

Cited by 59 publications

References 0 publications

Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept

Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept

Apolipoprotein B signal peptide polymorphism and plasma LDL-cholesterol response to low-calorie diet

Detection of putative functional angiotensinogen (AGT) gene variants controlling plasma AGT levels by combined segregation-linkage analysis

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