Standardized Flavonoid-Rich Eugenia jambolana Seed Extract Retards In Vitro and In Vivo LDL Oxidation and Expression of VCAM-1 and P-Selectin in Atherogenic Rats

Jadeja, Ravirajsinh N.; Thouaojam, Menaka C.; Sankhari, Jayantha M.; Jain, Mahendra; Devkar, Ranjitsinh V.; Ramachandran, A. V.

doi:10.1007/s12012-011-9140-0

Cited by 12 publications

(9 citation statements)

References 31 publications

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“…3). These observations are in accordance with our previous studies wherein, flavonoid and polyphenol rich herbal extracts have been reported to prevent ApoB fragmentation of Ox-LDL samples Jadeja et al 2011Jadeja et al , 2012. The ox-LDL induces pro-inflammatory changes in vascular endothelium and macrophage mediated uptake of ox-LDL transforms them into "lipid laden" foam cells (Steinberg 1997).…”

Section: Resultssupporting

confidence: 93%

Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

et al. 2014

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Several lines of evidences have established a lineage between Oxidised LDL (Ox-LDL) to apoptosis of macrophages in which the high level of intracellular cholesterol play a crucial role. This study assesses the potency of Murraya koenigii (MK) leaf extract in alleviating LDL oxidation and Ox-LDL induced lipotoxicity in murine macrophage (RAW 264.7) cells. Results indicated that presence of MK extract prevented oxidation of LDL as evidenced by its oxidation kinetics and formation of LDL oxidation products. Also, MK extract accounted for improvement in cell viability and mitochondrial membrane potential of Ox-LDL treated cells. The Ox-LDL induced increment in intracellular oxidative stress, nuclear condensation and apoptosis was effectively prevented by MK extract possibly due to their established anti-oxidant and free radical scavenging potentials which may be attributed to the presence of flavonoids present in the extract. Prevention of oxidative modification of LDL, free radical induced damage and Ox-LDL induced death of RAW 264.7 cells provide preliminary evidences of its antiatherosclerotic potential and warrants further elucidation and validation for its use in-vivo and may be useful as a functional food supplement and an alternative medicine to prevent LDL oxidation and oxidized LDL induced toxicity.

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Section: Resultssupporting

confidence: 93%

Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

et al. 2014

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“…34 These results are attributable to the potent anti-oxidant and free radical scavenging properties of ARCE or EJSE reported in our earlier studies. 20,21 Mitochondrion is the most vulnerable organelle under oxidative stress which plays a key role in apoptosis. 36 Mitochondrial injury triggers its permeability resulting in release of cytochrome C into the cytosol, a critical initiation step in ROS induced triggering of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…The total yield at the final step was 5.66% (w/w). 21 The total amount of flavonoids in EJSE was determined spectrophotometrically using quercetin as a standard. 23 Cell culture and treatment protocol.…”

Section: Preparation Of Anthocyanin Rich Red Cabbage Extract (Arce)mentioning

confidence: 99%

“…17,18 Reports on their phytochemical analysis have confirmed that their alcoholic extracts are rich in anthocyanins and flavonoids respectively 19,20 and have immense therapeutic potentials including anti-atherogenic properties as observed by us. 20,21 On the basis of these reports, it was thought pertinent to conduct a baseline study for assessment of cardioprotective potential of ARCE and EJSE. This inventory assesses the efficacy of ARCE and EJSE against hydrogen peroxide (H 2 O 2 ) induced cytotoxicity in H9C2 cells.…”

Section: Introductionmentioning

confidence: 99%

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Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

2012

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This study assesses the efficacy of anthocyanin rich Brassica olerecea leaves (ARCE) and flavonoid rich Eugenia jambolana seed (EJSE) extracts as possible cardioprotective agents against hydrogen peroxide (H(2)O(2)) induced cytotoxicity in H9C2 cells. Presence of ARCE or EJSE resulted in a superior cell viability and cell integrity as revealed by cell viability and lactate dehydrogenase release assays and acridine orange and ethidium bromide staining of control and H(2)O(2) treated H9C2 cells. These extracts were also able to reduce the impact of H(2)O(2) induced lipid peroxidation and depletion of intracellular glutathione. Also, there was an increase in mitochondrial membrane potential and reduced generation of intracellular reactive oxygen species following ARCE or EJSE treatments. These results suggest that ARCE and EJSE are capable of cardioprotective activity due to the high number of anthocyanins and flavonoids in them that are instrumental in lowering intracellular oxidative stress, preventing depletion of cellular antioxidants and improving cell viability.

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“…While the main flavonoid found in the pulp of S. cumini is anthocyanins, there are other important phytochemical constituents in the seeds of Jamun such as quercetin and rutin, and myricetin [ 57 ]. Among these compounds, quercetin, kaempferol, and rutin are mainly responsible for their effective bioactive potentials [ 71 ]. It is to be noted that the anthocyanins which are found in the fruit pulp are absent in the seeds, which might be affected during the ripening stage of the fruit [ 4 , 29 ].…”

Section: Clinical Effects Impartedmentioning

confidence: 99%

Major Bioactive Compounds from Java Plum Seeds: An Investigation of Its Extraction Procedures and Clinical Effects

Das

Nath

Talukdar

et al. 2023

Plants

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Java plum is widely recognized as a plant with valuable medicinal properties, originating from Indonesia and India and distributed globally in the tropic and sub-tropic regions of the world. The plant is rich in alkaloids, flavonoids, phenyl propanoids, terpenes, tannins, and lipids. The phytoconstituents of the plant seeds possess various vital pharmacological activities and clinical effects including their antidiabetic potential. The bioactive phytoconstituents of Java plum seeds include jambosine, gallic acid, quercetin, β-sitosterol, ferulic acid, guaiacol, resorcinol, p-coumaric acid, corilagin, ellagic acid, catechin, epicatechin, tannic acid, 4,6 hexahydroxydiphenoyl glucose, 3,6-hexahydroxy diphenoylglucose, 1-galloylglucose, and 3-galloylglucose. Considering all the potential beneficial effects of the major bioactive compounds present in the Jamun seeds, in the current investigation, the specific clinical effects and the mechanism of action for the major bioactive compounds along with the extraction procedures are discussed.

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Standardized Flavonoid-Rich Eugenia jambolana Seed Extract Retards In Vitro and In Vivo LDL Oxidation and Expression of VCAM-1 and P-Selectin in Atherogenic Rats

Cited by 12 publications

References 31 publications

Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

Flavonoid rich extract of Murraya Koenigii alleviates in-vitro LDL oxidation and oxidized LDL induced apoptosis in raw 264.7 Murine macrophage cells

Protective role of Brassica olerecea and Eugenia jambolana extracts against H2O2 induced cytotoxicity in H9C2 cells

Major Bioactive Compounds from Java Plum Seeds: An Investigation of Its Extraction Procedures and Clinical Effects

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