Standardization of chaga tincture and befungin

Abstract

Methods for standardizing the formulations Chaga Tincture and Befungin were developed, consisting of extraction of the non-phenol fraction predominantly containing tetracyclic triterpenes, including lanosterol and ergosterol, followed by spectrophotometry of colored complexes with vanillin in acidic conditions. The contents of tetracyclic triterpenes expressed as lanosterol ranged from 0.01% to 0.035% in Chaga Tincture and from 0.01% to 0.02% in Befungin.

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

“…In northwestern Russia, Siberia and Finland, the fruiting body of I. obliquus is decocted to treat cancer patients. At the year of 1955, the Medical Academy of Science in Moscow announced I. obliquus as anticancer substance, Ham et al, 2009;Lu et al, 2009 3β-Hydroxylanosta-8, 24-diene-21-al (4) Antimutagenic; (IC 50 , 232 mol ratio/32 pmol/TPA) antioxidant; hypoglycemic, Anticancer Ham et al, 2009;Lu et al, 2009;Chung et al, 2010 Lanosta-8, 23E-diene-3β, 22R, 25-triol (6) Antitumor promoting(IC 50 , 231 mol ratio/32 pmol/TPA) Taji et al, 2005 Lanosta-7, 9(11), 23E-triene-3β, 22R, 25-triol (8) Antitumor (Zhukovich et al, 2010).…”

Tetracyclic triterpenes from Inonotus Obliquus (Pers.) Pil. (Chaga) growing in Russia

“…In addition, the raw material could be standardized for content of tetracyclic triterpenes calculated as lanosterol in the purified non-phenolic fraction by a spectrophotometric method based on production of a colored complex of tetracyclic triterpenes with vanillin in acidic medium. It was found that the content of tetracyclic triterpenes calculated as lanosterol in Chaga tincture varied from 0.01 to 0.035%; in Befungin, 0.01 -0.02%; in the propyleneglycol extract, 0.02%; in the absolute dry raw material, 0.35 -0.8% [4].…”