Tetracyclic triterpenes from Inonotus Obliquus (Pers.) Pil. (Chaga) growing in Russia

Abstract

The tetracyclic triterpene content of Inonotus obliquus (Chaga) growing in Russia was investigated for the first time. Lanosterol, inotodiol, and ergosterol were isolated from the non-phenolic fraction. In addition, trametenolic acid was isolated from fraction III, which was prepared by extraction from diethylether solution with 5% NaOH.

Bioassay-Guided Isolation of DPP-4 Inhibitory Fractions from Extracts of Submerged Cultured of Inonotus obliquus

“…Nineteen proposed compounds were identified and are shown in Figure 2 . All the compounds have not been previously identified in I. obliquus [ 28 , 29 , 30 , 31 , 32 ]. There were ten alkaloid compounds: 3,3-dimethyl-9-(propylamino)-3,4-dihydro-1(2 H )-acridinone ( 1 ), 2-butyl-3-(3-methylphenyl)-4(3 H )-quinazolinone ( 2 ), 1-(4-methyl-1-piperazinyl)-2-{[3-(2-methyl-1-piperidinyl)propyl]amino}ethanone ( 3 ), 1-{[2-(diethylamino)ethyl]amino}-3-(4-methyl-1-piperazinyl)-2-propanol ( 4 ), N -{(1 S ,2 S )-1-benzyl-3-[1-(cyclohexylmethyl)hydrazino]-2-hydroxypropyl}- N 2-[(2-methoxyethoxy)carbonyl]-L-valinamide ( 5 ), 1,1-dimethyl-3,3-bis(2,2,6,6-tetramethyl-1-prop-2-en-1-ylpiperidin-4-yl)urea ( 6 ), 1-(3,6-dihydropyridin-1(2 H )-yl)-3-[3-(dimethylamino)propyl]urea ( 7 ), (2 R ,4 S ,5 S ,7 S )-5-amino- N -butyl-7-{4-[4-(dimethylamino)-butoxy]-3-(3-methoxypropoxy)benzyl}-4-hydroxy-2,8-dimethylnonanamide ( 8 ), 2,2-bis[2,2,6,6-tetramethyl-1-(octyloxy)piperidin-4-yl]-hexane-dioate ( 9 ) and 3-(4-cyclohexylbutyl)-6,11-dimethyl-1,2,3,4,5,6-hexahydro-2,6-methano-3-benzazocine ( 10 ).…”