Standardization of a non–heart-beating model in the rat for studying the mechanisms of renal cell death associated with cardiac arrest and preservation of the kidney

Bellemare, Sarah; Boucher, Anne; Dandavino, R; Marion, Alain; Dubé, Pascale; Pichette, Vincent; Hébert, Marie Josèe

doi:10.1016/s0041-1345(00)00824-1

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…In case of a DCD donation, cardiac arrest was induced by external compression of the heart (exogenous tamponade) until contractions ceased. Subsequently, the aorta and pulmonary artery were clamped close to the heart (to ensure a complete blood flow block), and the rat was kept at 37°C for 30 minutes . In case of non‐DCD donation, livers were procured immediately after laparotomy.…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the aorta and pulmonary artery were clamped close to the heart (to ensure a complete blood flow block), and the rat was kept at 378C for 30 minutes. (16) In case of non-DCD donation, livers were procured immediately after Non-DCD1NMP Non-DCD 3 hours NMP 2 hours reperfusion Non-DCD1SCS Non-DCD 3 hours SCS 2 hours reperfusion DCD1NMP DCD 3 hours NMP 2 hours reperfusion DCD1SCS DCD 3 hours SCS 2…”

Section: Procurement Of Dcd and Non-dcd Donor Liversmentioning

confidence: 99%

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Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

et al. 2016

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Bile duct injury may occur during liver procurement and transplantation, especially in livers from donation after circulatory death (DCD) donors. Normothermic machine perfusion (NMP) has been shown to reduce hepatic injury compared to static cold storage (SCS). However, it is unknown whether NMP provides better preservation of bile ducts. The aim of this study was to determine the impact of NMP on bile duct preservation in both DCD and non-DCD livers. DCD and non-DCD livers obtained from Lewis rats were preserved for 3 hours using either SCS or NMP, followed by 2 hours ex vivo reperfusion. Biomarkers of bile duct injury (gamma-glutamyltransferase and lactate dehydrogenase in bile) were lower in NMP-preserved livers compared to SCS-preserved livers. Biliary bicarbonate concentration, reflecting biliary epithelial function, was 2-fold higher in NMP-preserved livers (P < 0.01). In parallel with this, the pH of the bile was significantly higher in NMP-preserved livers (7.63 6 0.02 and 7.74 6 0.05 for non-DCD and DCD livers, respectively) compared with SCS-preserved livers (7.46 6 0.02 and 7.49 6 0.04 for non-DCD and DCD livers, respectively). Scanning and transmission electron microscopy of donor extrahepatic bile ducts demonstrated significantly decreased injury of the biliary epithelium of NMP-preserved donor livers (including the loss of lateral interdigitations and mitochondrial injury). Differences between NMP and SCS were most prominent in DCD livers. Compared to conventional SCS, NMP provides superior preservation of bile duct epithelial cell function and morphology, especially in DCD donor livers. By reducing biliary injury, NMP could have an important impact on the utilization of DCD livers and outcome after transplantation.

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Section: Methodsmentioning

confidence: 99%

Section: Procurement Of Dcd and Non-dcd Donor Liversmentioning

confidence: 99%

Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

et al. 2016

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“…The left kidney, renal vessels and ureter were isolated via a midline incision. After opening the thoracic cage, cardiac arrest was induced by manual cardiac tamponade for 5 min [17]. A ligature was placed superior to the left renal artery to prevent flushing of the right kidney, liver and intestine.…”

Section: Animals Materials and Methodsmentioning

confidence: 99%

Static cold storage preservation of ischemically damaged kidneys. A comparison between IGL-1 and UW solution

et al. 2008

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Summary Especially in damaged organs, adequate organ preservation is critically important to maintain viability. Institut Georges Lopez‐1 (IGL‐1) is a new preservation solution, with an extracellular sodium/potassium ratio and polyethylene glycol as a colloid. The influence of warm and cold ischemia was evaluated in a rat Lewis–Lewis transplant model with a follow up of 14 days. Eight groups of donation after cardiac death donor kidneys were studied with warm ischemia of 0 and 15 min followed by 0‐ or 24‐h cold storage (CS) preservation in IGL‐1 or UW‐CSS. Blood was collected daily during the first week and at day 14. Recipients were placed in metabolic cages at day 4 and 14 after transplantation allowing urine collection and adequate measurement of glomerular filtration rate. Focussing on inflammation, reactive oxygen species production, proximal tubule damage, proteinuria, histology, and renal function after transplantation we could not show any relevant difference between IGL‐1 and UW‐CSS. Furthermore, the combination of 15‐min warm ischemia and by 24‐h cold ischemia did not result in life sustaining kidney function after transplantation, irrespective of the used solution. In the present experiment, static CS preservation of ischemically damaged rat kidneys in either IGL‐1 or UW‐CSS rendered equal results after transplantation.

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“…Both kidneys and renal vessels were isolated via a midline incision. After opening the thoracic cage, cardiac arrest was induced by manual cardiac tamponade for 5 min [23]. Following cardiac arrest, a warm ischemic period of 0, 15 or 30 min was applied.…”

Section: Dcd Donor Proceduresmentioning

confidence: 99%

Deterioration of Endothelial and Smooth Muscle Cell Function in DCD Kidneys After Static Cold Storage in IGL-1 or UW

Maathuis

Groot

Ploeg

et al. 2009

Journal of Surgical Research

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Standardization of a non–heart-beating model in the rat for studying the mechanisms of renal cell death associated with cardiac arrest and preservation of the kidney

Cited by 4 publications

References 6 publications

Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

Normothermic machine perfusion reduces bile duct injury and improves biliary epithelial function in rat donor livers

Static cold storage preservation of ischemically damaged kidneys. A comparison between IGL-1 and UW solution

Deterioration of Endothelial and Smooth Muscle Cell Function in DCD Kidneys After Static Cold Storage in IGL-1 or UW

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