Static cold storage preservation of ischemically damaged kidneys. A comparison between IGL-1 and UW solution

Maathuis, Mark-Hugo J.; Ottens, Petra J.; Goor, Harry van; Zwaagstra, Jacco J.; Wiersema-Buist, Janneke; Schuurs, Theo A.; Ploeg, Rutger J.; Leuvenink, Henri G. D.

doi:10.1111/j.1432-2277.2007.00634.x

Cited by 13 publications

(12 citation statements)

References 36 publications

(38 reference statements)

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“…We chose 15 and 30 min for the duration of WI time in this animal model, as we had previously demonstrated that the combination of 15 min WI and 24 h CS results in a seriously damaged kidney graft, leading to delayed graft function after transplantation [9]. As NR is most interesting in the uncontrolled DCD (Maastricht categories I and II) [10] setting that will lead to severely damaged kidneys, we deliberately chose not to test the method on kidneys that have sustained only mild ischemic injury.…”

Section: Methodsmentioning

confidence: 99%

The effect of normothermic recirculation before cold preservation on post-transplant injury of ischemically damaged donor kidneys

Moers¹,

Rijt²,

Ploeg³

et al. 2011

Transplant International

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Summary Kidneys recovered from donation after cardiac death (DCD) are increasingly used to enlarge the deceased donor pool. Such renal grafts, especially those derived from uncontrolled DCD, have inevitably sustained profound warm ischemic injury, which compromises post‐transplant function. Normothermic recirculation (NR) of the deceased donor’s body before organ cooling could be an interesting approach to mitigate the detrimental effect of warm ischemia. To date, however, there is no evidence coming from preclinical studies to support the principle of NR in kidney transplantation. In this study, we subjected 48 Lewis rat kidneys to 15 or 30 min of warm ischemia, and subsequently 0, 1, or 2 h of NR. After 24 h cold storage, kidneys were transplanted into a recipient animal and 24 h later we measured the percentage of cortical necrosis, and determined gene expression of heme oxigenase‐1, heat shock protein‐70, transforming growth factor‐β, kidney injury molecule‐1, interleukin‐6, hypoxia inducible factor‐1α, monocyte chemoattractant protein‐1, and α‐smooth muscle actin in kidney tissue. We found that NR had no significant influence on any of these markers. Therefore, we conclude that this animal study by no means supports the presumed beneficial effect of NR on kidneys that have been severely damaged by warm ischemia.

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Section: Methodsmentioning

confidence: 99%

The effect of normothermic recirculation before cold preservation on post-transplant injury of ischemically damaged donor kidneys

Moers¹,

Rijt²,

Ploeg³

et al. 2011

Transplant International

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“…Previous studies [9,11,12,27] have also demonstrated the efficacy of PEG in liver, kidney, lung and pancreas preservation in experimental models and clinical trials. Recently PEG has been added to several newly developed preservation solutions, such as Polysol (PEG35 KDa, 20 g/L), SCOT (Solution de Conservation des Organes et des Tissus) solution (PEG20 KDa, 30 g/L), and IGL-1 (Institut Georges Lopez) solution (PEG35 KDa, 1 g/L) [6,10,13].…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of polyethylene glycol in University of Wisconsin preservation solution on human red blood cell aggregation and hemorheology

Zhao

Xiong

Yuan

et al. 2011

Clinical Hemorheology and Microcirculation

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Addition of hydroxyethyl starch (HES) to UW (University of Wisconsin) solution increases viscosity of the solution and red blood cell (RBC) aggregation. Recently, it was suggested that HES could be replaced by a new colloid, polyethylene glycol (PEG), in UW solution. The aim of this study was to see whether and how PEG affected RBC aggregation, and whether RBC aggregation parameters had any correlation with the molecular weight and concentration of PEG. After giving informed consent and signing consent documents, 12 healthy volunteers were enrolled in the study. Blood samples obtained from these volunteers were mixed with the test solutions with blood/solutions ratios of 5:1 and 1:1. Human RBC aggregation was investigated with an automatic hemorheological analyzer. Blood viscosity was measured with a cone-plate viscometer. Morphological characters of RBC aggregates were evaluated by light microscopy. It was found that viscosity was not affected by the Colloidfree UW solution. PEG20 kDa (1 and 10 g/L) and PEG35 kDa (1 g/L) had little effect on RBC aggregation, while PEG20 kDa (30 g/L) and PEG35 kDa (10 and 30 g/L) had a significant hyperaggregating effect on RBC. In conclusion, PEGs had a potential hyperaggregating effect on human RBC. This effect is correlated with PEG molecular weight and concentration. The use of large molecular weight and high concentration PEG in UW solution accounts for extended and accelerated aggregation of erythrocytes. The use of low concentration PEG35 kDa (1 g/L) would be the optimal choice.

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“…These characteristics render IGL-1 suitable for abdominal organ preservation. 7, 8, 9, 10, 11 However, the mechanisms by which IGL-1 protects against ischemia reperfusion (I/R) injury and maintains cell viability are unclear. Accumulating evidence suggested perturbations at the endoplasmic reticulum (ER) as novel subcellular effectors, possibly involved in the promotion of cell death during cold organ preservation.…”

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confidence: 99%

IGL-1 solution reduces endoplasmic reticulum stress and apoptosis in rat liver transplantation

Mosbah¹,

Zaoualí²,

Martel

et al. 2012

Cell Death Dis

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Injury due to cold ischemia reperfusion (I/R) is a major cause of primary graft non-function following liver transplantation. We postulated that I/R-induced cellular damage during liver transplantation might affect the secretory pathway, particularly at the endoplasmic reticulum (ER). We examined the involvement of ER stress in organ preservation, and compared cold storage in University of Wisconsin (UW) solution and in Institute Georges Lopez-1 (IGL-1) solution. In one group of rats, livers were preserved in UW solution for 8 h at 4 °C, and then orthotopic liver transplantation was performed according to Kamada's cuff technique. In another group, livers were preserved in IGL-1 solution. The effect of each preservation solution on the induction of ER stress, hepatic injury, mitochondrial damage and cell death was evaluated. As expected, we found increased ER stress after liver transplantation. IGL-1 solution significantly attenuated ER damage by reducing the activation of three pathways of unfolded protein response and their effector molecules caspase-12, C/EBP homologous protein-10, X-box-binding protein 1, tumor necrosis factor-associated factor 2 and eukaryotic translation initiation factor 2. This attenuation of ER stress was associated with a reduction in hepatic injury and cell death. Our results show that IGL-1 solution may be a useful means to circumvent excessive ER stress reactions associated with liver transplantation, and may optimize graft quality.

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Static cold storage preservation of ischemically damaged kidneys. A comparison between IGL-1 and UW solution

Cited by 13 publications

References 36 publications

The effect of normothermic recirculation before cold preservation on post-transplant injury of ischemically damaged donor kidneys

The effect of normothermic recirculation before cold preservation on post-transplant injury of ischemically damaged donor kidneys

In vitro effects of polyethylene glycol in University of Wisconsin preservation solution on human red blood cell aggregation and hemorheology

IGL-1 solution reduces endoplasmic reticulum stress and apoptosis in rat liver transplantation

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