2017
DOI: 10.1186/s40409-017-0101-3
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Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection

Abstract: BackgroundParacoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a cert… Show more

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Cited by 25 publications
(20 citation statements)
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“…PCM is the most prevalent chronic infection of the upper airdigestive tract in Latin America, whose importance is related to its severity, sequelae in spite of appropriate therapy, and repercussion on the quality of life [ 10 , 11 ]. Consistent with previous studies [ 12 , 13 , 14 , 15 ], most of our patients were men, adults, cigarette smokers and alcoholics who previously resided or currently reside in rural areas.…”
Section: Discussionsupporting
confidence: 91%
“…PCM is the most prevalent chronic infection of the upper airdigestive tract in Latin America, whose importance is related to its severity, sequelae in spite of appropriate therapy, and repercussion on the quality of life [ 10 , 11 ]. Consistent with previous studies [ 12 , 13 , 14 , 15 ], most of our patients were men, adults, cigarette smokers and alcoholics who previously resided or currently reside in rural areas.…”
Section: Discussionsupporting
confidence: 91%
“…It has advantage to be used by laboratories with few resources or even directly in the field. In addition, it presents high values of the accuracy parameters [ 354 ] (Table 4 ).…”
Section: Diagnosismentioning
confidence: 99%
“…1 Testes sorológicos específicos podem auxiliar no diagnóstico e na avaliação da resposta do hospedeiro ao tratamento. 8,9 O estudo anatomopatológico evidencia hiperplasia pseudoepiteliomatosa e infiltração supurativa granulomatosa, 9 com leveduras dentro e fora de células gigantes multinucleadas. A estrutura fúngica típica, denominada "roda de leme", corresponde a uma levedura arredondada e de membrana espessa, contendo um ou mais brotos com base estreita.…”
Section: Discussionunclassified