Staining of Tilia Mucilages with Mayer's Tannic Acid-Ferric Chloride

Pizzolato, Thompson Demetrio

doi:10.2307/2484311

Cited by 46 publications

(23 citation statements)

References 2 publications

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“…For histochemical analysis, different treatments were performed to highlight the major chemical classes in the composition of the secretion: ruthenium red for acidic mucilage (Gregory & Baas 1989), tannic acid and ferric chloride for mucilage (Pizzolato 1977), periodic acid-Schiff's (PAS) reaction (pararosaniline C.I. 42500) for carbohydrates (McManus 1948), Sudan Black (C.I.…”

Section: Methodsmentioning

confidence: 99%

Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae)

Monteiro

Demarco

2017

Acta Bot. Bras.

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Flowers of Asclepiadoideae are notable for possessing numerous nectaries and elaborate coronas, where nectar can accumulate but is not necessarily produced. Given the complexity and importance of these structures for reproduction, this study aimed to analyze the ontogeny of the corona, the structure and position of nectaries and the histochemistry of the nectar of species of Asclepiadeae. Two types of coronas were observed: androecial [C(is)] and corolline (Ca). Th e development of the C(is)-type of corona initiates opposite the stamens in all species examined with the exception of Matelea in which it begins to develop as a ring around the fi lament tube. Despite their morphological variation, coronas typically originate from the androecium. A notable diff erence among the studied species was the location of the nectaries. Primarily, they are located in the stigmatic chamber, where nectar composed of carbohydrates and lipids is produced. A secondary location of nectaries found in species of Peplonia and Matelea is within the corona, where nectar is produced and stored, composed of carbohydrates and lipids in Peplonia and only carbohydrates in Matelea. Th e functional role of nectar is related to the location of its production since it is a resource for pollinators and inducers of pollen germination.

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Section: Methodsmentioning

confidence: 99%

Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae)

Monteiro

Demarco

2017

Acta Bot. Bras.

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“…For permanent staining, the sections were deparaffinized in xylene and rehydrated in a decreasing ethanol series. Next, they were placed sequentially in 1% tannic acid, 3% iron chloride (Pizzolato 1977), 1% safranin and 2% astra blue (Gerlach 1969). The permanent sections were mounted with Apathy's syrup (sucrose and gum arabic) (Richards 1943).…”

Section: Methodsmentioning

confidence: 99%

The role of root buds in the regeneration of Casearia sylvestris Swartz (Salicaceae) in the cerrado, São Carlos, São Paulo state, Brazil

Imatomi

Souza

Gualtieri

et al. 2014

Hoehnea

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-(The role of root buds in the regeneration of Casearia sylvestris Swartz (Salicaceae) in the Cerrado, São Carlos, São Paulo State, Brazil). The present work describes the fire effects on the population structure of Casearia sylvestris Swartz (Salicaceae). Diameter and height structure as well as spatial distribution patterns of the root sprouts were analyzed pre-and post-fire. The morphology and anatomy of the underground system were also analyzed in order to investigate the nature and origin of sprouts from this system. In this study, a 10-ha of Cerrado area was sampled from February to November 2006; in August, a fire burned roughly 80% of the study area, allowing us to compare the data collected pre-and post-fire. Our findings show that the underground systems consisted of specialized roots and buds originated close to the vascular cambium. The spatial distribution of the root sprouts became clustered pre-and post-fire and most of the root sprouts were concentrated in the first size class, indicating that the sprouts from roots play an important role in the recovery of this species in burnt areas. Keywords: Cerrado, natural fires, sprouting, vegetative reproduction RESUMO -(A função de raízes gemíferas na regeneração de Casearia sylvestris Swartz (Salicaceae) no Cerrado, São Carlos, SP, Brasil). Este artigo descreve os efeitos de incêndio na estrutura populacional de Casearia sylvestris Swartz (Salicaceae). Foram analisados a estrutura de diâmetro e altura e o padrão de distribuição espacial antes e após o fogo. Além disso, foi realizada a caracterização morfológica e anatômica do sistema subterrâneo para determinar a natureza e origem dos brotos oriundos desse sistema. Neste estudo foram amostrados 10 ha de fevereiro a novembro de 2006, em agosto, um incêndio queimou cerca de 80% da área de estudo, permitindo a comparação de dados coletados antes e após este evento. Foram verificados que os sistemas subterrâneos gemíferos são de natureza radicular e que os brotos se originam próximo ao câmbio vascular. A distribuição espacial dos brotos de raiz tornou-se agregada após o incêndio e houve maior concentração de brotos de raiz na primeira classe de tamanho indicando que esses brotos são responsáveis pela recomposição dessa espécie em áreas queimadas. Palavras-chave: brotamento, Cerrado, incêndios naturais, reprodução vegetativa

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“…Los tratamientos realizados para evidenciar los principales componentes de las paredes de los laticíferos y del látex fueron: rojo de rutenio para pectinas (Johansen, 1940) y mucílagos ácidos (Gregory y Baas, 1989), ácido tánico y cloruro férrico para mucílago (Pizzolato, 1977), reacción PAS (Periodic-Acid-Schiff's reagent; pararosanilina C.I. 42500) para polisacáridos (Jensen, 1962), reactivo de Lugol para almidón (Johansen, 1940), azul de anilina (C.I.…”

Section: Materiales Y Métodosunclassified

Micromorphology and Histochemistry of the Laticifers from Vegetative Organs of Asclepiadoideae species (Apocynaceae)

Demarco¹

2014

Acta biol. colomb.

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Staining of Tilia Mucilages with Mayer's Tannic Acid-Ferric Chloride

Cited by 46 publications

References 2 publications

Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae)

Corona development and floral nectaries of Asclepiadeae (Asclepiadoideae, Apocynaceae)

The role of root buds in the regeneration of Casearia sylvestris Swartz (Salicaceae) in the cerrado, São Carlos, São Paulo state, Brazil

Micromorphology and Histochemistry of the Laticifers from Vegetative Organs of Asclepiadoideae species (Apocynaceae)

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