Staining

Sharma, Arun Kumar; Sharma, A.

doi:10.1016/b978-0-408-70942-2.50010-9

Cited by 6 publications

(1 citation statement)

References 157 publications

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“…Ploidy level itself can be approached by manual chromosome analysis using staining method (Sharma and Sharma, 1980) or using ploidy analyzer machines (Nwakanma et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genome identification of bananas (Musa L.) from East Java Indonesia assessed with PCR-RFLP of the Internal Transcribed Spacers Nuclear Ribosomal DNA

2015

Int. J. Biosci.

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East Java Province has high diversity of local banana cultivars with various local names and diverse morphological characteristics. The genome identification of those banana cultivars are needed to provide valid identity of the banana accessions to be used as basic data for in-situ and/or ex-situ banana conservation management and for further breeding banana material. Polymerase Chain Reaction -Restriction Fragment Length Polymorphism (PCR-RFLP) of the Internal Transcribed Spacer (ITS) region nuclear ribosomal Deoxyribo Nucleic Acid (nrDNA) using RsaI restriction endonuclease enzyme was conducted to 68 banana accessions collected from 17 Regencies in East Java Province. The RsaI digested fragments showed consistent polymorphic banding DNA patterns; in M. acuminata the wild species and cultivars produced two fragments of 530 bp and 120 bp whereas M. balbisiana species and its hybrid cultivars produced three fragments of 350 bp, 180 bp and 120 bp. From this study it was found that about 45 accessions were identified as M. acuminata species (AA/AAA genomes). It comprises of 3 wild species M. acuminata (AAw), 17 M. acuminata diploid cultivars (AA) and 25 M. acuminata triploid cultivars (AAA). Wild species M. balbisiana identified only one accession (BBw), and the hybrid cultivars comprises of 14 M acuminata × balbisiana (ABB) cultivars and 8 M. acuminata × balbisiana (AAB) cultivars. Clustering analysis result based on restriction fragment ITS region showed that BBw, ABB and AAB genome groups were close related and clustered as sister group whilst AA/AA genome group became the out-group with similarity coefficient between 0,635 to 1.00.

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“…Ploidy level itself can be approached by manual chromosome analysis using staining method (Sharma and Sharma, 1980) or using ploidy analyzer machines (Nwakanma et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Genome identification of bananas (Musa L.) from East Java Indonesia assessed with PCR-RFLP of the Internal Transcribed Spacers Nuclear Ribosomal DNA

2015

Int. J. Biosci.

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Anionic peroxidase production byArnebia euchromacallus

Farhadi¹,

Haghbeen²,

Marefatjo³

et al. 2011

Biotech and App Biochem

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Arnebia euchroma callus, obtained from the root cell culture of an Iranian native specimen, has gained a doubling time of 63 H after regular subculturing on Linsmaier-Skoog (LS) medium containing sugar (50 g/L), 2,4-dichlorophenoxyacetic acid (10(-6) M), and kinetin (10(-5) M) under darkness at 25°C. Despite the observed somaclonal variations, peroxidase production by the A. euchroma calli has been stable over 4 years under the aforementioned conditions. Isoelectric focusing experiments revealed that the partially purified A. euchroma peroxidases (AePoxs) are mainly anionic with pI values of about 5.5 and 6.6. AePox reaches its optimal activity at 55°C and pH 7.5. Results of the various kinetic studies suggest that AePox belongs to the type III plant peroxidases with no activity for the oxidation of 3-indoleacetic acid, but seems to play a role in the lignin biosynthesis and H(2) O(2) regulation during the proliferation of the A. euchroma cells on LS medium. Comparing the biochemical properties of AePox with horseradish peroxidase and in view of the ease of solid cell culture, the A. euchroma callus could be considered as a source of plant peroxidase for some biotechnological applications.

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