Micropropagation of Anethum graveolens L Through Axillary Shoot Proliferation

Sharma, Rajnish; Wakhlu, A. K.; Boleria, M.

doi:10.1007/bf03263214

Cited by 14 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Role of cytokinins is obvious in bud proliferation and shoot multiplication (Aggarwal et al 1999;Pereira et al 1999;Kaur et al 2000;Sharma et al 2004;Kaloo et al 2013) and the stimulating effect of BA on multiple shoot formation has been reported for several medicinal and aromatic plant species (Pattnaik and Chand 1996;Saxena et al 1997;Khalafalla and Hattori 1999;Faisal et al 2006;Kaur et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

A protocol for in vitro propagation was developed for Viola pilosa, a plant of immense medicinal value. To start with in vitro propagation, the sterilized explants (buds) were cultured on MS basal medium supplemented with various concentrations of growth regulators. One of the medium compositions MS basal+0.5 mg/l BA+0.5 mg/l TDZ+0.5 mg/l GA 3 gave best results for in vitro shoot bud establishment. Although the problem of shoot vitrification occurred on this medium but this was overcome by transferring the vitrified shoots on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The same medium was found to be the best medium for further in vitro shoot multiplication. 100 % root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 1 mg/l IBA. In vitro formed plantlets were hardened and transferred to soil with 83 % survival. Additionally, conservation of in vitro multiplying shoots was also attempted using two different approaches namely slowing down the growth at low temperature and cryopreservation following vitrification. At low temperature retrieval rate was better at 10°C than at 4°C after conservation of in vitro multiplying shoots. In cryopreservation-vitrification studies, the vitrified shoot buds gave maximum retrieval of 41.66 % when they were precooled at 4°C, while only 16.66 % vitrified shoots were retrieved from those precooled at 10°C. Genetic stability of the in vitro grown plants was analysed by RAPD and ISSR markers which indicated no somaclonal variation among in vitro grown plants demonstrating the feasibility of using the protocol without any adverse genetical effects.

show abstract

Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…The role of antioxidant enzymes during organogenesis and somatic embryogenesis in some species have been studied in recent years (Mathur et al 2002a,b, 2008, Dutta Gupta and Datta 2003, Meratan et al 2009, Misra et al 2010, Sharifi and Ebrahimzadeh 2010. There are few reports on micropropagation of A. graveolens through axillary shoots (Sharma et al 2004), indirect regeneration through leaf explants (Jana and Shekhawat 2010b), but to the best of our knowledge, it is the first report on Anethum graveolens regeneration from nodal explants and biochemical parameters studied in vitro.…”

Section: Introductionmentioning

confidence: 88%

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Jana¹,

Shekhawat²

2012

Biologia plant.

View full text Add to dashboard Cite

An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 µM N 6 -benzyladenine (BA) and 0.21 µM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 µM BA and 1.85 µM kinetin. The average length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ¼ MS augmented with 4.9 µM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased. Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that regenerated plants are true to type plants.

show abstract

“…1e). Many authors have reported IBA as the best rooting auxin for Anethum graveolens (Sharma et al, 2004), Thapsia garganica (Makunga et al, 2006), Anisochilus carnosus (Jeyachandran, 2004) Annona sequamosa (Roxana, 2005), Psudarthria viscida (Vinothkumar et al, 2009 and2010) and Quisqualis indica (Poornima and Shivamurthy, 2005). At higher concentrations of auxins profuse callus was produced at the basal end of microshoots which inhibited the growth and elongation of roots.…”

Section: Resultsmentioning

confidence: 99%

Clonal propagation of <i>Wattakaka volubilis</i> through nodal explant culture

Vinothkumar¹,

Murugavelh

Senthikumar³

2011

Ceylon J. Sci. (Biol. Sci.)

View full text Add to dashboard Cite

A protocol was developed for microporpagation of Wattakaka volubils (L.f) Stapf, an important medicinal plant in India. In vitro shoot multiplication nodal explants of W. volubilis was achieved on solid MS basal medium supplemented with BAP (6-Benzylaminopurine) and NAA (Naphthalene acetic acid). Presence of NAA in the culture medium along with BAP promoted multiplication of shoots than BAP alone. The highest shoot multiplication rate (23.4±0.48) was observed after 28 days of culture on MS basal medium supplemented with 0.6 mg/l BAP and 0.2 mg/l NAA. Elongated shoots produced roots within 6 weeks in half strength MS basal medium supplemented with 0.6 mg/l IBA (Indole-3-butyric acid) and 3% (w/v) sucrose. Rooted plantlets were acclimatized with a survival rate of 95% and transferred to the greenhouse and thereafter successfully transferred to the field with 90% survival rate.

show abstract

Micropropagation of Anethum graveolens L Through Axillary Shoot Proliferation

Cited by 14 publications

References 9 publications

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Clonal propagation of <i>Wattakaka volubilis</i> through nodal explant culture

Contact Info

Product

Resources

About