In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Jana, Sonali; Shekhawat, G. S.

doi:10.1007/s10535-012-0009-2

Cited by 31 publications

(8 citation statements)

References 35 publications

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“…Embryogenic callus induction was inhibited in higher concentration of auxin and cytokinin in accordance with Jana and Shekhawat (2012) results. Also, results of Dhir and Shekhawat (2013) showed that increasing the cytokinins concentration in regeneration medium decreased the percentage of responding.…”

Section: Somatic Embryogenesis and Histological Studies Of Somatic Emsupporting

confidence: 86%

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Amini

Deljou

Nabiabad

2016

Physiol Mol Biol Plants

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Developmental deficiency of somatic embryos and regeneration to plantlets, especially in the case of transformation, are major problems of somatic embryo regeneration in alfalfa. One of the ways to overcome these problems is the use of natural plant regulators and nutrients in the culture medium of somatic embryos. For investigating the influence of Cuscuta campestris extract on the efficiency of plant regeneration and transformation, chimeric tissue type plasminogen activator was transferred to explants using Agrobacterium tumefaciens, and transgenic plants were recovered using medium supplemented with different concentration of the extract. Transgenic plants were analyzed by PCR and RT-PCR. Somatic embryos of Medicago sativa L. developed into plantlets at high frequency level (52 %) in the maturation medium supplemented with 50 mg 1 -1 C. campestris extract as compared to the medium without extract (26 %). Transformation efficiency was 29.3 and 15.2 % for medium supplemented with dodder extract and without the extract, respectively. HPLC and GC/MS analysis of the extract indicated high level of ABA and some compounds such as Phytol, which can affect the somatic embryo maturation. The antibacterial assay showed that the extract was effective against some strains of A. tumefaciens. These results have provided a scientific basis for using of C. campestris extract as a good natural source of antimicrobial agents and plant growth regulator as well, that can be used in tissue culture of transgenic plants.

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Section: Somatic Embryogenesis and Histological Studies Of Somatic Emsupporting

confidence: 86%

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Amini

Deljou

Nabiabad

2016

Physiol Mol Biol Plants

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“…1d and e), with approximately 89 % of plantlets surviving the transition from tissue culture to the field. In comparison, the survival rate of A. graveolens plantlets was 60 %, and that of A. xanthorrhiza 62.5 % (Sliva et al 2010;Jana and Shekhawat 2012). The in vitromicropropagated plants grew well in the experimental plot, were morphologically uniform, and produced seed in the second season of vegetative growth ( Fig.…”

Section: Rooting Of Shoots and Plant Transfer Into Soilmentioning

confidence: 97%

“…Shoot proliferation decreased significantly under higher concentrations of BA, or when no IAA was present. Higher concentrations of BA (whether applied alone, or in combination with auxin) are known to decrease the number of new microshoots per explant in other for 12 months Apiaceae such as Thapsia garganica (Makunga et al 2003), E. foetidum (Gayatri et al 2006), Crithmum maritimum (Grigoriadou and Maloupa 2008), Arracacia xanthorrhiza (Sliva et al 2010), and Anethum graveolens (Jana and Shekhawat 2012). In contrast, the efficiency of shoot proliferation in Centella asiatica, and Vanasushava pedata was seen to increase in concentrations of up to 5.0 mg L -1 BA (Tiwari et al 2000;Karuppusamy et al 2006).…”

Section: Shoot Induction and Proliferationmentioning

confidence: 99%

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Thiem

Kikowska

Krawczyk³

et al. 2013

Plant Cell Tiss Organ Cult

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A protocol for in vitro production of genetically uniform populations of the medicinal plant Eryngium planum, rich in selected phenolic acids, has been established. Shoot-tips were collected from axenic seedlings and grown on a Murashige and Skoog basal medium supplemented with 6-Benzyladenine (BA) and Indole-3-acetic acid (IAA). The highest shoot proliferation efficiency (17 shoots per explant) was obtained when 1.0 mg L -1 BA and 0.1 mg L -1 were added. Proliferating shoots were rooted and transferred to soil (89 % frequency of survival). Flow cytometric analysis of intact (field-grown) and microrpropagated plants revealed that all plants were uniform in genome size and had similar DNA contents. Thin-layer chromatography (TLC) analysis indicated that multiple shoots and roots from in vitro-derived plants produced high amounts of phenolic acids, primarily of rosmarinic acid (RA). Levels of phenolic acids in in vitroderived plants were similar to those of intact plants. Furthermore, high-performance liquid chromatography revealed that root cultures in liquid medium accumulated substantial levels of RA. Thus, rapid establishment of in vitro-grown organ cultures of E. planum can also serve as reliable sources for bioactive compounds.

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“…2). Positive changes in CAT activity have often been observed in relation to mild water stress [21,25,35]. Thus increment in SOD and CAT activity can be considered as the protection against ROS possibly generated during acclimatization.…”

Section: Antioxidative Enzyme Activitiesmentioning

confidence: 99%

Synthetic seed production and physio-biochemical studies inCassia angustifoliaVahl. — a medicinal plant

Bukhari¹,

Siddique²,

Perveen³

et al. 2014

Acta Biologica Hungarica

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Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl 2 • 2H 2 O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 µM) + indole-3-acetic acid (1.0 µM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1-6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 µM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (P N ), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.

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In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

Cited by 31 publications

References 35 publications

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Improvement of in vitro embryo maturation, plantlet regeneration and transformation efficiency from alfalfa (Medicago sativa L.) somatic embryos using Cuscuta campestris extract

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Synthetic seed production and physio-biochemical studies inCassia angustifoliaVahl. — a medicinal plant

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