Synthetic seed production and physio-biochemical studies inCassia angustifoliaVahl. — a medicinal plant

Abstract

Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl 2 • 2H 2 O were found most suitable for encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium supplemented with thidiazuron (5.0 µM) + indole-3-acetic acid (1.0 µM) produced maximum number of shoots (10.9 ± 0.78) after 8 weeks of culture exhibiting (78%) in vitro conversion response. Encapsulated nodal segments demonstrated successful regeneration after different period (1-6 weeks) of cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots when cultured on half strength Murashige and Skoog medium supplemented with 1.0 µM indole-3-butyric acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were successfully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months old in vitro-raised plant was compared with in vivo seedlings of the same age. Changes in the content of photosynthetic pigments, net photosynthetic rate (P N ), superoxide dismutase and catalase activity in C. angustifolia indicated the adaptation of micropropagated plants to ex vitro conditions.

Regeneration of plants from alginate-encapsulated axenic nodal segments ofPaederia foetidaL. - A medicinally important and vulnerable plant species

“…In the present study, the shoots generated from sprouted synthetic seeds (those who failed to germinate) were rooted successfully on plant growth regulator free ½ MS. Similar observation of rooting were also reported in Albizia lebbeck (Perveen and Anis 2014), Bacopa monnieri (Rency et al 2017), Cassia angustifolia (Bukhari et al 2014), Cineraria maritime (Srivastava et al 2009), Nyctanthes arbortrisits (Jahan and Anis 2015), Ruta graveolens (Ahmad et al 2012) and Withania somnifera (Fatima et al 2013). Acclimatization of in vitro raised plants is always considered as a critical stage due to possibilities of high percentages of plant loss during transplantation (Pospóšilová et al 1999;Ahmad et al 2012).…”

Diuretic and antioxidant activities of the aqueous extract of leaves of Cassia occidentalis (Linn.) in rats

“…Interestingly, experimental evidence supports the applications in traditional medicine. Phytochemical studies of C. occidentalis leaves revealed the presence of a number of pharmacologically active families of molecule, including tanins, saponins, cardiac glycosides, terpenoides and anthroquinones, terpenes, and inorganic elements [7,8]. Extracts of this plant were reported antifungal, antiviral [9,10], antibacterial, anthelmintic [11,12], antispasmodic, analgesic, antipyretic, anti-inflammatory [13,14], and hepatoprotective properties in humans and experimental models [15,16].…”

In Vitro Mid-Term Conservation of Acorus calamus L. via Cold Storage of Encapsulated Microrhizome

“…3A). Synthetic seeds were also produced by encapsulation of in vitro grown vegetative propagules in calcium alginate beads in many species i.e., Acacia hybrid 22 , Cassia angustifolia 23 , etc. They can be used like real seeds for transport, storage and plant production.…”