Stable knockdown of heparanase expression in gastriccancer cells in vitro

Zheng, Liduan; Jiang, Guosong; Pu, Jiarui; Dong, Jing; Hou, Xiaohua; Tong, Qiangsong

doi:10.3748/wjg.15.5442

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…These apparently contradictory results may be reconciled by the observation that an increased number of heparanase‐positive tumour cells were inversely correlated with a four‐fold decrease in tumour cell proliferation. A common finding by others is that heparanase can enzymatically influence cell migration and invasiveness, but not proliferation; 41–43 for example, in a stable heparanase knockdown model in gastric cancer, migration and invasiveness were reduced, but not tumour cell proliferation 44 . Further studies also clearly showed that heparanase‐expressing cells were able to activate neighbouring tumour and non‐tumour cells (such as endothelial cells), with subsequent improved cell migration and invasiveness, but no notable increase in proliferation 45 .…”

Section: Discussionmentioning

confidence: 95%

Heparanase expression in head and neck squamous cell carcinomas is associated with reduced proliferation and improved survival

et al. 2011

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Heparanase expression in head and neck squamous cell carcinomas is associated with reduced proliferation and improved survivalAims: Cellular expression of heparanase, a degrading enzyme of the extracellular matrix, is associated with poorer prognosis in several cancers. The present analysis, has studied the role of heparanase in tumour growth and clinical outcome in patients with head and neck squamous cell carcinoma (HNSCC). Methods and Results: We analysed the cellular expression of the active form of heparanase in 71 human HNSCCs, using immunohistochemistry. The results were compared with clinicopathological data and, in 65 cases with immunoreactivity for the proliferation marker, MIB1. Cellular heparanase expression was detected in 41 of 71 (57.74%) cases; in particular, UICC IV-stage tumours showed high heparanase levels. Heparanase was localized mainly in the cytoplasm and, to a lesser extent, at the cell membrane. High levels of heparanase were significantly correlated with an almost four-fold decrease in MIB1 labelling (P = 0.006). Comparison with clinical outcome by multivariate analysis revealed that patients with high-level heparanase expression had prolonged overall survival (P = 0.029). Conclusions: Although heparanase was mainly found in late-stage HNSCCs, cellular heparanase expression in HNSCCs was associated with prolonged overall survival. We propose that the proliferation-reducing effect of high heparanase levels might outweigh the tumourpromoting effects of heparanase, especially in advanced tumours.

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Section: Discussionmentioning

confidence: 95%

Heparanase expression in head and neck squamous cell carcinomas is associated with reduced proliferation and improved survival

et al. 2011

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“…Heparanase overexpression in gastric cancer has been correlated with tumour invasion and metastasis 55–57. In contrast, silencing of heparanase expression blocked the growth, invasion and metastasis of gastric cancer cells 58 59…”

Section: Discussionmentioning

confidence: 96%

Expression of heparan sulfate in gastric carcinoma and its correlation with clinicopathological features and patient survival

Thike

Tan

et al. 2010

J Clin Pathol

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“…revealed that heparanase involved in the tumor invasion and metastasis of gastric cancer in vivo (20). Conversely, stable knockdown of heparanase expression can efficiently inhibit the invasiveness, metastasis and angiogenesis of human gastric cancer cells (25). Considering the results from our present study along with those of the studies mentioned before, we consider that heparanase expression might play an important role in inducing a poor outcome in patients with gastric cancer by promoting invasive and metastatic activities of gastric cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of SATB1 and heparanase in gastric cancer and its relationship to clinicopathologic features

Cheng¹,

Lu²,

Wang³

et al. 2010

APMIS

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The purpose of this study was to investigate the expression of special AT-rich binding protein 1 (SATB1) and heparanase in human gastric cancer as well as its relationship to the clinicopathologic factors. Specimens from 102 patients who underwent radical gastrectomy between 2000 and 2002 were studied by immunohistochemistry for SATB1 and heparanase expression. SATB1 and heparanase were positively expressed in 48.0% and 51.0% of gastric cancer cases, respectively. The expression of SATB1 and heparanase was significantly correlated with the depth of invasion, tumor-node-metastatsis (TNM) stage, lymph node metastasis, whereas SATB1 expression was also significantly correlated with distant metastasis. Patients with SATB1-negative expression and heparanase-negative expression had higher survival rates than those with SATB1-positive or heparanase-positive expression. Moreover, a positive correlation was found between SATB1 and heparanase. In multivariable analysis, SATB1 expression was also identified as an independent prognostic indicator for gastric cancer. Our results suggest that combined analysis of SATB1 and heparanase expression may have significant value in determining invasion and metastasis of gastric cancer and assessing prognosis in patients with gastric cancer.

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Stable knockdown of heparanase expression in gastriccancer cells in vitro

Cited by 11 publications

References 32 publications

Heparanase expression in head and neck squamous cell carcinomas is associated with reduced proliferation and improved survival

Heparanase expression in head and neck squamous cell carcinomas is associated with reduced proliferation and improved survival

Expression of heparan sulfate in gastric carcinoma and its correlation with clinicopathological features and patient survival

Expression of SATB1 and heparanase in gastric cancer and its relationship to clinicopathologic features

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