Stable-Isotope Dilution GC-MS for Determination of Toluene in Submilliliter Volumes of Whole Blood

Jones, A. D.; Dunlap, Michael R.; Gospe, Sídney M.

doi:10.1093/jat/18.5.251

Search citation statements

Order By: Relevance

Paper Sections

Select...

Methods1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1994

2007

Publication Types

Select...

Article3

Book2

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 8 publications

(1 citation statement)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A heparinized blood specimen was obtained by cardiac puncture and was frozen for later determination of toluene concentration by gas chromatographylmass spectroscopy (26). The gravid uterus and maternal liver were then removed and weighed.…”

Section: Methodsmentioning

confidence: 99%

The Effects of High-Dose Toluene on Embryonic Development in the Rat

et al. 1994

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

The Effects of High-Dose Toluene on Embryonic Development in the Rat

et al. 1994

View full text Add to dashboard Cite

Drug deposition in adipose tissue and skin: evidence for an alternative source of positive sweat patch tests

Levisky

Bowerman

Jenkins

et al. 2000

Forensic Science International

View full text Add to dashboard Cite

A comparison of 1H8-and 2H8-toluene toxicokinetics in men

Pierce¹

1999

Xenobiotica

View full text Add to dashboard Cite

1. To examine the bioequivalence of an isotope-labelled tracer to study toxicant disposition, we conducted 33 controlled human exposures to a mixture of 50 ppm 1H8-toluene and 50 ppm 2H8-toluene for 2 h, and measured concentrations in blood and breath, and metabolite levels in urine for 100 h post-exposure. 2. A physiologically based kinetic (PBK) model found that compared with 1H8-toluene, 2H8-toluene had a 6.4+/-13% (mean+/-SD) lower AUC, a 6.5+/-13% higher systemic clearance (1.46+/-0.27 versus 1.38+/-0.25 l/h-kg), a 17+/-22% larger terminal volume of distribution (66.4+/-14 versus 57.2+/-10 l/kg) and a 9.7+/-26% longer terminal half-life (38+/-12 versus 34+/-10 h) (p < 0.05 for all comparisons). 3. The higher 2H8-toluene clearance may have been due to an increased rate of ring oxidation, consistent with the 17% higher observed fraction of 2H5- versus 1H5-cresol metabolites in urine. 4. The larger terminal volume and half-lives for 2H8-toluene suggested a higher adipose tissue/blood partition coefficient. 5. Observed isotope differences were small compared with interindividual differences in 1H8-toluene kinetics from previous studies. 6. The PBK model allowed us to ascribe observed isotope differences in solvent toxicokinetics to underlying physiologic mechanisms.

show abstract

Stable-Isotope Dilution GC-MS for Determination of Toluene in Submilliliter Volumes of Whole Blood

Cited by 8 publications

References 11 publications

The Effects of High-Dose Toluene on Embryonic Development in the Rat

The Effects of High-Dose Toluene on Embryonic Development in the Rat

Drug deposition in adipose tissue and skin: evidence for an alternative source of positive sweat patch tests

A comparison of 1H8-and 2H8-toluene toxicokinetics in men

Contact Info

Product

Resources

About