Stable Formation of Fruiting Body inCordyceps bassiana

Abstract

In order to breed a Cordyceps bassiana isolate that stably forms fruiting body in artificial cultivation, isolates derived from subculturing and single spores were tested through mating. From C. bassiana EFCC 783, three subcultured isolates EFCC 2830, EFCC 2831 and EFCC 2832 were obtained and fourteen single conidial isolates were obtained from these three subcultured isolates. Two different morphological types were found in the fourteen single conidial isolates. One type was able to form synnemata and another type was not able to form synnemata. Since switch of morphological type was not observed despite their continuous subculturing, cross was performed between the two types and the formation of fruiting body was examined. Ascospores were obtained from a selected fruiting body formed by hybrid of the cross. Self-cross and combinational cross of the ascospore-derived isolates generated hybrids that stably produce high quality fruiting body in artificial media.

Heterothallic Type of Mating System forCordyceps cardinalis

“…Cultural studies should be conducted more critically to identify the mating system for Cordyceps . C. bassiana shows a very unstable mating system in culture [10, 11]. In nature, Cordyceps species generally show variation in their morphological characteristics.…”

Successful Development ofCordyceps bassianaStromata fromBeauveria bassiana

“…Single conidial isolates of B. bassiana EFCC 13188 were similar to C. bassiana EFCC 12511 isolates [6] with regard to colony pigmentation and texture. Three combinations of isolates of B. bassiana EFCC 13188-1 × 5, 3 × 8, and 5 × 8 produced perithecial stromata (Table 1, Fig.…”

“…All of the eight single conidial isolates of B. bassiana EFCC 13188 and four single ascospore isolates of C. bassiana EFCC 12511a × b [6] were inoculated in brown rice medium in all possible combinations of two isolates at a time to observe the nature of fruiting bodies (Table 2). The fruiting bodies were observed for perithecia development after fifty days of incubation.…”

“…Shimazu et al [1] have successfully begun the process of discovering teleomorphs from anamorphs by incubating them under controlled environmental conditions. Recently, C. bassiana has been reported from Korea and has been successfully produced in culture [6, 7]. …”

Cultural Characteristics and Fruiting Body Production inCordyceps bassiana

“…The second species, C. bassiana , was described as growing from a Lepidopteran cadaver in the wild, from China [3, 4]. Recently, C. bassiana has been successfully grown in vitro , with the aim of developing it for commercial use [5-7]. …”