Cultural Characteristics and Fruiting Body Production inCordyceps bassiana

Abstract

Single ascospore isolates of Cordyceps bassiana were observed for their colony pigmentation on Sabouraud Dextrose agar plus Yeast Extract (SDAY) plates and were inoculated in a brown rice medium for production of fruiting bodies. Colony pigmentation did not show any relationship with perithecial stromata formation. The isolates were also grown on opposite sides of SDAY agar plates and were observed for vegetative compatibility. Neither vegetative compatibility nor perithecial stromata could be found to be related to each other. It was concluded that fertile fruiting body production was independent of colony pigmentation and vegetative compatibility. Synnemata formation was found to be more common than perithecial stromata formation. This might be due to its highly conidiogenous anamorphic stage, i.e., Beauveria bassiana.

Growth and Cultural Characteristics ofCordyceps cardinalisCollected from Korea

“…However, all media induced an almost compact mycelial density. Organic nitrogen sources are important nutritional factors for mycelial growth of entomopathogenic fungi [21, 22, 24], which could be due to their insect hosts.…”

“…Cultures of Cordyceps species are usually established from ascospores, and the germination rates and growth rates differ depending on the species. For example, C. bassiana , C. bifusispora , C. militaris , C.pruinosa , C. scaraebaeicola , M. yongmunensis , O. pentatomae , and Shimizuomyces paradoxa grow quickly, while C. nakazawai , O. gracilis and O. heteropoda grow more slowly [22-26]. C. martialis , C. ochraceostromata , C. ramosopulvinata , C. rosea , O. longissima , O. sphecocephala , and O. yakushimensis grow very slowly [25, 26].…”

“…Conidiation differs by species in Cordyceps [25, 27, 28], and microcycle conidiation has been reported in several Cordyceps species [25, 27]. Colonies of Cordyceps species also show different types of pigmentations in culture; C. militaris has yellowish white to orange pigmentation, C. pruinosa is red with white sectors, M. yongmunensis is greenish white, while C. bassiana does not develop pigmentation [19, 21, 22, 24]. Fruiting body production under artificial conditions has been reported recently for C. cardinalis [29].…”

Heterothallic Type of Mating System forCordyceps cardinalis

“…Cordyceps sinensis ), have played very important roles in the economic development of local communities [1, 2]. Recently, the mycelium growth and fruiting body formation of Cordyceps species have been studied with great interest with the objective of large-scale cultivation [3-11]. Mating system is an important genetic factor for fruiting body formation in fungi [12-14].…”

First report of the occurrence of Ophiocordyceps melolonthae (Ascomycota: Hypocreales: Ophiocordycipitaceae) in larvae of Diloboderus abderus Sturm (Coleoptera: Melolonthidae) in Brazil

“…Progress in methods for in vitro fruiting of Cordyceps species with Beauveria anamorphs are promising for developmental studies of Beauveria and its Cordyceps teleomorphs through integrated phylogenetics, developmental and mating studies (Sung et al 2006, Lee et al 2010, Rehner et al 2011.…”