Growth and Cultural Characteristics ofCordyceps cardinalisCollected from Korea

Abstract

Cordyceps cardinalis was reported in Japan and the USA in 2004, and its fruiting bodies have recently been cultured in Korea. Herbarium specimens preserved at the Cordyceps Research Institute, Mushtech, Korea were revised and identified as C. cardinalis, based on morphological characters and conidial structures. Most of the C. cardinalis specimens were collected from Mt. Halla in Jeju-do. The effects of various nutritional sources and environmental conditions such as temperature and pH on mycelial growth of C. cardinalis were studied. Oatmeal agar, Martin's peptone dextrose agar, and Schizophyllum (mushroom) genetics complete medium plus yeast extract resulted in the best mycelial growth. Among carbon sources, cereals, and nitrogen sources, maltose, oatmeal, and peptone resulted in the best mycelial growth respectively. Mineral salts helped to increase growth rate but only resulted in thin mycelial density, similar to water agar. A temperature of 25℃ and a pH of 7 resulted in the highest mycelial growth. Based on these results, a Cordyceps cardinalis composite medium (CCM) was formulated with 1% maltose, 2% oatmeal, 1% peptone, and 2% agar. Use of the CCM resulted in slightly better mycelial growth than that of other commonly used agar media. Only organic nitrogen sources imparted a reddish pigmentation to the agar media, but this character diminished after several subcultures. A 7 day culture duration resulted in the best mycelial growth.

Growth and Cultural Characteristics ofOphiocordyceps longissimaCollected in Korea

“…This result indicates that MD and CD of the isolates do not correlate with each other on agar culture. Czapek-dox agar (CDA) produced a thin density and the shortest CD in O. longissima isolates, as reported in other Cordyceps species [26, 27]. CDA does not contain an organic nitrogen source, which may be why it could not support rich growth of the O. longissima isolates.…”

Isolation, identification, culture and production of adenosine and cordycepin from cicada larva infected with entomopathogenic fungi in Thailand

“…These results are in general agreement with those of Nam et al (2006), who found that C. sphecocephala grew more slowly on PDA medium than PDBLA or PDBAA media containing of frozen dried honeybee larvae and honeybee adult. Supplementing media with mineral salts has also been shown to increase growth rate of C. cardinalis (Sung et al, 2010).…”

Vegetative Development of <i>Sparassis crispa</i> in Various Growth Conditions and Effect of Electric Pulse Simulation on Its Fruit Body Production

“…This temperature is reported as the most favourable for the majority of mushrooms which include Coprinus comatus [15], Paecilomyces hepiali [12], Paecilomyces fumosoroseus [14] [16], Ophiocordyceps heteropoda [17] and Cordyceps cardinalis [18]. The next favourable temperature was 20˚C.…”