Successful Development of<i>Cordyceps bassiana</i>Stromata from<i>Beauveria bassiana</i>

Lee, Jeo; Shrestha, Bhushan; Sung, Gi‐Ho; Han, Sang-Kuk; Sung, Jae-Mo

doi:10.4489/myco.2010.38.1.013

Cited by 10 publications

(5 citation statements)

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“…This fungus produces anti-bacterial and anti-fungal compounds [ 13 ]. In the context of growing studies on Cordyceps and allied species [ 14 - 23 ], isolates of this species were tested for growth on different agar media, at different temperatures, and under light and dark conditions. This species showed moderate growth on agar media with the possibility of growing to a larger scale under optimum cultural conditions and nutrition sources.…”

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Cultural Characteristics ofOphiocordyceps heteropodaCollected from Korea

Sung

Shrestha²,

Han

et al. 2011

Mycobiology

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Isolates of Ophiocordyceps heteropoda (Kobayasi) collected from Mt. Halla on Jeju-do, Korea were tested for mycelial growth on different agar media and in the presence of different carbon and nitrogen sources. Similarly, isolates were also incubated at different temperatures as well as under continuous light and dark conditions. Growth was better on Hamada agar, basal medium, and malt-yeast agar, but poor on Czapek-Dox agar. Different carbon sources such as dextrin, saccharose, starch, lactose, maltose, fructose, and dextrose resulted in better growth. Complex organic nitrogen sources such as yeast extract and peptone revealed the most effective growth. Mycelial growth was best at 25℃. The growth rate was faster in the dark than the light, but mycelial density was less compact in the dark.

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Cultural Characteristics ofOphiocordyceps heteropodaCollected from Korea

Sung

Shrestha²,

Han

et al. 2011

Mycobiology

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“…Besides fruiting body formation, mating type can also help resolve biological species concepts in fungi [22]. In addition to ascospore-derived isolates, conidial isolates of asexual fungi have also led to the development of sexual states of Cordyceps in culture by proper combination of opposite mating types [23]. …”

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Heterothallic Type of Mating System forCordyceps cardinalis

Sung

Shrestha²,

Han

et al. 2010

Mycobiology

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Cordyceps cardinalis successfully produced its fruiting bodies from multi-ascospore isolates. However, subcultures of multi-ascospore isolates could not produce fruiting bodies after few generations. Fruiting body production also differed from sector to sector of the same isolate. Single ascospore isolates were then co-inoculated in combinations of two to observe the fruiting characteristics. Combinations of certain isolates produced perithecial stromata formation, whereas other combinations did not produce any fruiting bodies. These results show that C. cardinalis is a heterothallic fungus, requiring two isolates of opposite mating types for fruiting body production. It was also shown that single ascospore isolates are hermaphrodites.

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“…To obtain the transcriptome, RNA from Bb8028 was isolated from three different sources: (1) 5-day old mycelia grown on rice medium; (2) proto fruiting bodies (stromata like-structures) obtained from a cross between Bb8028 and Bb5078 co-cultivated for 2 months on rice medium as described [18]; and (3) infected mosquitoes that had just died. Rice medium was composed of 60 g of brown rice and 10 g of dried silkworm pupae, and 100 ml of distilled water.…”

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Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

et al. 2016

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BackgroundEntomopathogenic fungi such as Beauveria bassiana are promising biological agents for control of malaria mosquitoes. Indeed, infection with B. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. Natural isolates of B. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. In this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three RNA libraries, and applied a genome comparison approach to uncover genetic mechanisms underpinning virulence.ResultsA high-quality, near-complete genome assembly was achieved for the highly virulent isolate Bb8028, which was compared to the assemblies of the four other isolates. Whole genome analysis showed a high level of genetic diversity between the five isolates (2.85–16.8 SNPs/kb), which grouped into two distinct phylogenetic clusters. Mating type gene analysis revealed the presence of either the MAT1–1–1 or the MAT1–2–1 gene. Moreover, a putative new MAT gene (MAT1-2–8) was detected in the MAT1–2 locus. Comparative genome analysis revealed that Bb8028 contains 163 genes exclusive for this isolate. These unique genes have a tendency to cluster in the genome and to be often located near the telomeres. Among the genes unique to Bb8028 are a Non-Ribosomal Peptide Synthetase (NRPS) secondary metabolite gene cluster, a polyketide synthase (PKS) gene, and five genes with homology to bacterial toxins. A survey of candidate virulence genes for B. bassiana is presented.ConclusionsOur results indicate several genes and molecular processes that may underpin virulence towards mosquitoes. Thus, the genome sequences of five isolates of B. bassiana provide a better understanding of the natural variation in virulence and will offer a major resource for future research on this important biological control agent.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3339-1) contains supplementary material, which is available to authorized users.

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Successful Development ofCordyceps bassianaStromata fromBeauveria bassiana

Cited by 10 publications

References 5 publications

Cultural Characteristics ofOphiocordyceps heteropodaCollected from Korea

Cultural Characteristics ofOphiocordyceps heteropodaCollected from Korea

Heterothallic Type of Mating System forCordyceps cardinalis

Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes

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