Stable Colonization of Orally Administered Lactobacillus casei SY13 Alters the Gut Microbiota

Abstract

The gut microbiota plays an important role in intestinal health. Probiotics such as Lactobacillus are known to regulate gut microbes and prevent diseases. However, most of them are unable to colonize their stability in hosts’ intestinal tracts. In this study, we investigated the ability of Lactobacillus casei SY13 (SY13) to colonize the intestinal tract of BALB/c mice, after its oral administration for a short-term (once for a day) and long-term (once daily for 27 days) duration. Furthermore, we also evaluated the influence of its administration on the gut microbial structure and diversity in mice. Male BALB/c mice were gavaged with 108 colony-forming units (CFU) of SY13, and TaqMan-MGB probe and Illumina MiSeq sequencing were performed to assess the colonization ability and bacterial community structure in the cecum contents. The results showed that long-term treatment with SY13 enhanced its ability to form a colony in the intestine tract in contrast to the short-term treatment group, whose colony was retained for only 3 days. Oral administration of SY13 also significantly enhanced the gut microbial diversity. Short-term treatment with SY13 (SSY13) elevated Firmicutes and diminished Bacteroidetes phyla compared with long-term treatment (LSY13) and controls. The findings laid the foundation for the study of probiotic colonization ability and improvement of microbiota for the prevention of gut diseases.

Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models

“…The health benefits of probiotics are diminished mainly due to the substantial reduction of viable probiotic bacteria under the harsh conditions in the gastrointestinal cavity and the colonization resistance caused by commensal bacteria [52]. In a previous study aimed to evaluate the colonization ability of L. casei SY13 and explore its effects on gut microbial structure and diversity in mice, the authors found that the stable colonization of L. casei SY13 was associated with dosage and treatment days, and thus laid a foundation for studying interactions between L. casei SY13 and other members of the gut microbiota [53]. The long-lasting retention period in the intestinal tract is necessary for B. lactis BL-99 to play its functions in the intestinal health and the disease prevention and treatment.…”

Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis

“…Different results were obtained through long-term vs. short-term oral supplementation of probiotics that seem also to exert differential effects on diversity and community structure of microbiota. In other studies, authors showed that short-term and long-term clinical studies alter the diversity and community structure of intestinal microbiota in mice with different physiological effects [ 85 ]. Long-term oral of L. casei sy13 enhanced the ability of colonization in the intestinal tract, however, a single time of oral dose had a greater effect on gut microbiota structure at phylum and genus.…”

Metabolite secretions of Lactobacillus plantarum YYC-3 may inhibit colon cancer cell metastasis by suppressing the VEGF-MMP2/9 signaling pathway

“…Lactic acid bacteria (LAB), such as Lactobacillus and Bifidobacterium , are also members of the gut microbiome [ 8 – 11 ]. Some metabolites secreted by LAB exhibit antibacterial properties, for example, bacteriocin; these metabolites may be potent substitutes for antibiotics [ 12 , 13 ].…”