Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models

Abstract

This study was designed to explore the therapeutics and the mechanisms of a patented and marked gastric acid and intestine juice-resistant probiotics Bifidobacterium lactis BL-99 (B. lactis BL-99) on the intestinal inflammation and functions in the zebrafish models. After feeding for 6 hours, B. lactis BL-99 was fully retained in the larval zebrafish intestinal tract and stayed for over 24 hours. B. lactis BL-99 promoted the intestinal motility and effectively alleviated aluminum sulfate-induced larval zebrafish constipation (p < 0.01). Irregular high glucose diet induced adult zebrafish intestinal functional and metabolic disorders. After fed with B. lactis BL-99, IL-1β gene expression was significantly down-regulated, and IL-10 and IL-12 gene levels were markedly up-regulated in this model (p < 0.05). The intestinal lipase activity was elevated in the adult zebrafish intestinal functional disorder model after B. lactis BL-99 treatment (p < 0.05), but tryptase content had no statistical changes (p > 0.05). B. lactis BL-99 improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and up-and-down metabolites were markedly recovered after treatment of B. lactis BL-99 (p < 0.05). These results suggest that B. lactis BL-99 could relieve intestinal inflammation and promote intestinal functions, at least in part, through modulating intestinal and microbial metabolism to maintain intestinal health.

Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

“…Bacillus coagulans 09•712 and Lactobacillus plantarum 08•923 showed robust zebrafish gut adhesion capability, and played immunoregulatory and immunoprotective roles in effective stimulation of anti-inflammatory response and barrier regeneration within the mucosa to protect zebrafish against infection (53). Bifidobacterium lactis BL-99 also could promote the intestinal integrity, improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and recover microbiota metabolism to maintain intestinal health (54). Besides, several studies have found Pediococcus could exert beneficial effects in zebrafish (55)(56)(57)(58).…”

Effects of grafting on chemical constituents, toxicological properties, antithrombotic activity, and myocardial infarction protection of styrax secreted from the trunk of Liquidambar orientalis Mill

“…Gene expressions were detected by qRT-PCR using iTaq Universal SYBR Green (Bio-Rad, Hercules, CA, USA) on the CFX Connect Real-Time PCR Detection System (Bio-Rad, CA, USA). The primer sequences were referred to other studies and listed in Table 1 [33][34][35][36][37][38][39][40][41]. The relative expression levels of the genes were normalized to β-actin and calculated using the 2 -ΔΔCT method.…”

Prolonged efficacy of Bifidobacterium lactis in the treatment of functional dyspepsia

“…11 Then, it was con rmed that BL-99 could maintain intestinal health by reducing intestinal in ammation. 12 Moreover, recent studies suggested that BL-99 could utilize fructo-oligosaccharide (FOS) to regulate the composition and structure of gut microbiota in patients with constipation, thus promoting the production of short chain fatty acids (SCFAs). 13 Hence, the prolonged e cacy of BL-99 to improve FD symptoms and the underlying mechanism were explored by a pilot randomised, positive-drug and placebo-controlled trial.…”