Metabolite secretions of Lactobacillus plantarum YYC-3 may inhibit colon cancer cell metastasis by suppressing the VEGF-MMP2/9 signaling pathway

Abstract

Background Colorectal cancer (CRC) is a major clinical challenge, and the gut microbiome plays important roles in the occurrence and metastasis of CRC. Lactobacillus and their metabolites are thought to be able to suppress the growth of CRC cells. However, the antimetastatic mechanism of Lactobacillus or their metabolites toward CRC cells is not clear. Therefore, the aim of this study was to assess the inhibitory mechanism of cell-free supernatants (CFSs) of L. rhamnosus GG, L. casei M3, and L. plantarum YYC-3 on metastasis of CRC cells. Results YYC-3 CFS showed the highest inhibitory effect on CRC cell growth, invasion and migration, and inhibited MMP2, MMP9, and VEGFA gene and protein expression, and protein secretion. Furthermore, it suppressed the activities of MMPs by gelatin zymography. Moreover, the effective compounds in these CFSs were analyzed by Q Exactive Focus liquid chromatography–mass spectrometry. Conclusions Our results showed that metabolite secretions of YYC-3 may inhibited cell metastasis by downregulating the VEGF/MMPs signaling pathway. These data suggest that treatment of CRC cells with metabolites from L. plantarum YYC-3 may reduce colon cancer metastasis.

Novel targets in rectal cancer by considering lncRNA–miRNA–mRNA network in response to Lactobacillus acidophilus consumption: a randomized clinical trial

“…They showed that pre-inoculation with L. acidophilus in Bulb/c mice resulted in retarding tumor volume growth, lowered histopathology scores, enhanced apoptosis of tumor cells, and down-regulated surface proteins' expression 25 . Besides, Yue et al found that the metabolites of L. acidophilus could suppress the cell metastasis of colon cancer by inhibiting the VEGF/MMPs signaling pathway 28 . Agah et al showed that L. acidophilus could induce a lower level of CEA and CA19-9 and a higher level of IFN-γ in the azoxymethane-induced colon cancer by altering the T-Cell signature to increase CD4+ and CD8+ cells 10 , 29 .…”

Microbiome Analysis Reveals the Attenuation Effect of Lactobacillus From Yaks on Diarrhea via Modulation of Gut Microbiota

“…Animal gut microbiota is one of the largest and most complicated existing micro-ecosystems that provides an important barrier to bacterial infections ( Lynch and Pedersen, 2016 ; Li et al., 2020 ; Liu et al., 2020b ). Additionally, it helps in providing mucosal immunity, material metabolism, and nutrient absorption and regulation ( Wu and Wu, 2012 ; Yue et al., 2020 ). Generally, ongoing competition and interaction of microorganisms may gradually change in microbial community structure from simple to a complicated and eventually a dynamic and balanced ecosystem ( Jami et al., 2013 ; Zhao et al., 2015 ).…”

Anticancer Potential of Post-Fermentation Media and Cell Extracts of Probiotic Strains: An In Vitro Study

“…The concentrations of the PFM are the most often given as a percentage or mg/mL, less often in µg/mL. The range of the tested concentrations is from 0.47% to 100% [ 11 , 35 , 36 , 37 , 38 , 39 ] or from 0.0001 to 1000 mg/mL [ 18 , 29 , 31 , 34 , 40 , 41 ]. In all of the above-mentioned studies, the MTT assay dominates as a tool of antiproliferative activity testing for cancerous cell lines and cytotoxicity assayed to normal cells.…”