Stabilization of α-chymotrypsin by covalent immobilization on amine-functionalized superparamagnetic nanogel

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…Thus, the immobilized enzyme was proved to possess the abilities of resisting a wide range of pH values and temperature. This might result from the multipoint interactions arising from covalent conjugation of the enzyme molecule on the carboxyl-functionalized MNPs as well as diffusional limitations of the immobilized molecules [27].…”

Nano-Immobilized Biocatalysts for Biodiesel Production from Renewable and Sustainable Resources

“…The strong binding of enzyme to the support matrix via the covalent bond prevents enzyme leaching from the surface and improves the thermal stability in some cases [18,22]. This technique, however, often provokes the deactivation of enzyme because of the conformational restriction of the enzyme by covalent binding [22,26]. Hong et al [26] immobilized α-chymotrypsin on an amine-functionalized superparamagnetic nanogel by covalent binding and compared the stability of the immobilized enzyme and free enzyme.…”

“…This technique, however, often provokes the deactivation of enzyme because of the conformational restriction of the enzyme by covalent binding [22,26]. Hong et al [26] immobilized α-chymotrypsin on an amine-functionalized superparamagnetic nanogel by covalent binding and compared the stability of the immobilized enzyme and free enzyme. The optimal pH value of the free enzyme and the immobilized enzyme was at pH 7.8, and the pH profile was similar.…”

Hydrolysis of Egg Yolk Protein in a Packed Bed Reactor by Immobilized Enzyme

“…Alkaline protease 를 담체에 고정화 시키는 방법도 일반적인 효소의 경우와 같 은데 고정화 효소는 불용성 물질에 효소를 흡착하는 방법 [3,23], 젤 상태의 물질에 효소를 가두는 방법 [9,17,21], 고분자 의 상태에 따라 sol/gel상태로 고정화 방법 [21,22], 이중의 작 용기를 갖는 시약에 상호 가교하는 방법 [2], 그리고 불용성 담 체에 효소를 공유 결합 하는 방법 [10,15,18] (Fig. 3).…”