Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

Screening of lipase inhibitors from Scutellaria baicalensis extract using lipase immobilized on magnetic nanoparticles and study on the inhibitory mechanism

“…In our previous study, we have reported the detailed characteristics of lipase-immobilized MNPs. The storage stability of immobilized lipase was significantly better than the free form at 20°C within 20 days, and it retained about 55 % of the initial activity after being used for six times [35]. [36][37][38], compounds 1, 2, and 3 are identified as baicalin, wogonin, and oroxylin A, respectively.…”

Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk

“…Magnetic nanoparticles (MNPs) have unique properties including high affinity to proteins, capable of being modified with reactive functional groups for chemical modifications, good biocompatibility, easy and convenient isolation from reaction mixture and recycling for reuse (Zhu et al, 2014). Despite all these http://dx.doi.org/10.1016/j.foodchem.2016.06.011 0308-8146/Ó 2016 Elsevier Ltd. All rights reserved.…”

Micron-sized flower-like Fe3O4@GMA magnetic porous microspheres for lipase immobilization

“…Firstly, magnetic microsphere is easy to be modified (like silane coupling agent) due to abundant functional groups on the surface, and good biocompatibility [13][14][15] ; for another, because of the property of rapid separation, it improves the reusability of immobilized enzyme and reduces production costs. Further, Zhu et al [6] utilized succinic anhydride modified co-precipitation Fe 3 O 4 nanoparticles to immobilize porcine pancreatic lipase. Further, Zhu et al [6] utilized succinic anhydride modified co-precipitation Fe 3 O 4 nanoparticles to immobilize porcine pancreatic lipase.…”