Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk

Atacan, Keziban; Çakıroğlu, Bekir; Özacar, Mahmut

doi:10.1016/j.foodchem.2016.06.011

Cited by 131 publications

(54 citation statements)

References 39 publications

(44 reference statements)

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“…The reaction happened between the NH 2 group found in the enzyme protein molecule and the free C=O group located on GA (the cross-linker) forming C=N-bond as reported by Yuan et al (2016). Magnetic nano-particles have unique properties as high affinity to proteins and ability to be chemically modified with reactive functional groups (Atacan et al 2016). Misson et al (2015) reported that, polymeric nano-carrier can be fabricated easily in nanometer scale with a large surface area in the range of 30-500 nm.…”

Section: Discussionmentioning

confidence: 85%

Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

et al. 2020

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Background: α-Amylase randomly hydrolyzes starch molecule to produce oligosaccharides of different chain length. It is among the most significant hydrolytic enzymes used in industrial applications. Enzyme immobilization is the simplest way to solve the stability problem of protein under industrial harsh conditions. Magnetic nano-particles considered suitable for immobilization due to their unique characteristics. The polymer nanocarriers still the feature of modifiable surfaces of carriers for further conjugation with biomolecule. This study aims to promote the immobilization of Bacillus subtilis MK1 α-amylase using the statistical optimization of the chemical modification of the chitosan-magnetic nano-magnetic particle beads and their ability to apply. Results: B. subtilis MK1 α-amylase was successfully immobilized on chitosan-magnetic nano-particles using a method combining the advantages of both physical adsorption and covalent binding. The beads were chemically modified using polyethyleneimine (PEI) followed by glutaraldehyde (GA). Aminated beads by (PEI), activated beads by (GA), and immobilized enzyme on activated beads were characterized using FTIR. Morphological examinations of the beads surface before and after conjugation with the α-amylase enzyme were carried out using scanning electron microscope (SEM). Chemical modification parameters of the beads were optimized using response surface methodology based on central composite design. Statistical approach enhanced the immobilization yield (IY%) by 1.5-fold. The application of immobilized enzyme in the baking process enhanced dough-raising about 2.3-fold and can be reused for 5 cycles with 100% activity. Conclusions: Statistical methods are an important way to improve immobilization yield and efficiency. The ANOVA data confirmed the fitness of the model which possessed R 2 value (0.975) and the adjusted R 2 value (0.940). The results confirm the ability to reuse the immobilized enzyme in industrial processes.

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Section: Discussionmentioning

confidence: 85%

Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

et al. 2020

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“…Trametes versicolor laccase was immobilized onto polyvinyl alcohol microspheres activated with aldehyde and used as a biosensor. The immobilized enzymes exhibited good chemical and storage stability and reusability (Bai et al 2014). Horseradish peroxidase was immobilized, covalently through its lysine residues, on a N -hydroxysuccinimidyl carbonate-activated poly(2hydroxyethyl methacrylate) (PHEMA) brush (Lane et al 2011).…”

Section: Synthetic Polymersmentioning

confidence: 99%

The Enzyme Immobilization: Carriers and Immobilization methods

Spasojević¹,

Prodanović²,

Pantić³

et al. 2020

JEPM

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Strategies based on the enzyme application are increasingly replacing the conventionalchemical procedures because of their efficiency, quicker performance and environmentalprotection. However, natural enzymes can rarely be used in industrysince their beneficial features cannot endure the industrial conditions. Additionaldrawbacks of natural enzymes are their inhibition by reaction products and difficultyto be removed from the reaction mixture. The most promising techniqueto substantially improve the enzyme properties, such as activity, pH, thermal andorganic-solvent stability, reusability and storage stability, in non-natural environmentsis by the enzyme immobilization. In this review different techniques usedto immobilize enzymes to inert carriers were summarized. Different materials ofboth the organic and inorganic origin were used as carriers for the enzyme immobilization.A class of new materials where the enzyme performance was enhancedby combining different classical materials and shaping in specific forms was alsosummarized.

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“…The surface modification of SPIONs with different biological agents can NPs improve their properties, including stability, water solubility, biocompatibility, and cellular uptake, which are highly needed for biomedical applications, such as drug delivery, imaging, and biosensors. Different kinds of amino acids, sugars, proteins, polymers, and nucleic acids are used for modification of NPs.…”

Section: Introductionmentioning

confidence: 99%

“…In some cases, incorporation of magnetic NPs could improve bioactivity of the scaffolds, these magnetite NPs enhance the biological properties of cells that are needed for bone regeneration including cell mineralization. 30 The surface modification of SPIONs with different biological agents can NPs improve their properties, including stability, water solubility, biocompatibility, and cellular uptake, [31][32][33] which are highly needed for biomedical applications, such as drug delivery, imaging, and biosensors. Different kinds of amino acids, 34 sugars, 35,36 proteins, 37 polymers, 38,39 and nucleic acids 40 are used for modification of NPs.…”

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confidence: 99%

Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering

Nazari

Heirani‐Tabasi

Hajiabbas

et al. 2019

J of Cellular Biochemistry

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Mimicking the structure of extracellular matrix (ECM) of myocardium is necessary for fabrication of functional cardiac tissue. The superparamagnetic iron oxide nanoparticles (SPIONs, Fe3O4), as new generation of magnetic nanoparticles (NPs), are highly intended in biomedical studies. Here, SPION NPs (1 wt%) were synthesized and incorporated into silk‐fibroin (SF) electrospun nanofibers to enhance mechanical properties and topography of the scaffolds. Then, the mouse embryonic cardiac cells (ECCs) were seeded on the scaffolds for in vitro studies. The SPION NPs were studied by scanning electron microscope (SEM), X‐ray diffraction (XRD), and transmission electron microscope (TEM). SF nanofibers were characterized after incorporation of SPIONs by SEM, TEM, water contact angle measurement, and tensile test. Furthermore, cytocompatibility of scaffolds was confirmed by 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay. SEM images showed that ECCs attached to the scaffolds with elongated morphologies. Also, the real‐time PCR and immunostaining studies approved upregulation of cardiac functional genes in ECCs seeded on the SF/SPION‐casein scaffolds including GATA‐4, cardiac troponin T, Nkx 2.5, and alpha‐myosin heavy chain, compared with the ones in SF. In conclusion, incorporation of core‐shells in SF supports cardiac differentiation, while has no negative impact on ECCs' proliferation and self‐renewal capacity.

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Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk

Cited by 131 publications

References 39 publications

Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

The Enzyme Immobilization: Carriers and Immobilization methods

Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering

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