Hydrolysis of Egg Yolk Protein in a Packed Bed Reactor by Immobilized Enzyme

Abstract

Alkaline protease for the hydrolysis of egg yolk protein was immobilized on five carriers -Duolite A568, Celite R640, Dowex-1, Dowex 50W and Silica gel R60. Duolite A568 showed a maximum immobilization yield of 24.7%. Optimum pH for the free and immobilized enzyme was pH 8 and 9, respectively. However, no change was observed in optimum temperature (50 o C). Thermal stability was observed in immobilized enzymes compared to free enzymes. The immobilized enzyme retained 86% activity after 10 cycle operations in a repeated batch process. The effect of flow rate on the stability of enzyme activity in continuous packed-bed reactor was investigated. Lowering flow rate increased the stability of the immobilized enzyme. After 96 hr of continuous operation in a packed-bed reactor, the immobilized enzyme retained 83 and 61% activity when casein and egg yolk were used as a raw materials, respectively.

Analysis of an Immobilized β-Galactosidase Reactor with Competitive Product Inhibition Kinetics

“…고정화 효소에 대한 반응기로는 충진층 (packed bed) 반응기, 유동층 반응기 그리고 막(membrane) 반응기 등이 있다 [11,26,27]. 이 중에서 충진층 반응기가 널리 사용되는데 고정화 효소에 대한 높은 전환율을 이룰 수 있으 며, 반응기의 운전 비용이 저렴하고 그리고 연속 운전이 가능 한 장점이 있다 [13,14]. 단점으로는 온도 조절이 어렵고 유체 의 흐름의 불균형으로 인한 채널링 현상이 발생할 수 있다 [5].…”

Hydrolysis of Cellulose by Immobilized Cellulase in a Packed Bed Reactor

“…효소 고정 화의 단점인 기질의 물질전달에 의한 확산 저항을 감소시키기 위한 연구도 진행되었다 [1,26]. 고정화 효소 반응기로는 충진 층 반응기, 유동층 반응기, 그리고 막 반응기 등이 사용되었다 [10,12].…”