Stability of chromatophores during ATP regeneration in an enzyme reactor

Cofactor Regeneration for Enzyme-Catalysed Synthesis

“…A review of efforts up to 1981-82 cites instability as a major problem (Ochiai, Tanaka and Fukui, 1983). More recent work has investigated this difficulty (Matsuoka, Suzuki and Aizawa, 1981;Smeds and Enfors, 1986) as well as the influence of external factors on the function of organelles (Laretta-Garde and Thomas, 1987). Although it is unlikely that regeneration systems based on chloroplasts will be applied in the near future, the concept of using photosynthetic energy to regenerate ATP is an attractive idea, deserving further research.…”

“…Several good reports describe or review chemical routes to nucleotides (Scheit, 1980;Davisson et al, 1987;Hobbs, 1987). Immobilized derivatives of ATP (Mosbach, Larsson and Lowe, 1976;Wang and King, 1979;Yamazaki and Maeda, 1981;Berke et al, 1984;Smeds and Enfors, 1986) have proved less useful in enzyme-catalysed synthesis than similarly immobilized nicotinamide cofactors. ATP-utilizing enzymes tolerate the immobilized cofactors less well than NAD(P)(H)-utilizing enzymes, exhibiting greater reductions in catalytic activity.…”