A simple method for isolation of high-molecular-weight DNA from Rhodotorula gracilis

Vishnuvardhan, Daesety; Joseph, Richard W.

doi:10.1007/bf00367101

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“…Similarly, cells (100 mg) were treated with crude lytic enzyme (2 ml) obtained from the fungus Paecilomyces lilacinus. The crude enzyme was obtained according to the method of Vishnuvardhan and Joseph (1995). The cells were treated with 0·5% chitosan in 1% acetic acid for 30 min at 30°C (Jaspers et al 1975).…”

Section: Other Permeabilization Methodsmentioning

confidence: 99%

A comparative study on permeabilization treatments for in situ determination of phytase of Rhodotorula gracilis

Somashekar

Joseph

1998

Lett Appl Microbiol

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Rhodotorula gracilis, a red yeast, was shown to produce phytase and the cultural conditions were standardized for phytase production. Permeabilization treatments using detergents, organic solvents, enzymes and physical methods were performed and compared by measuring the phytase activity of whole cells. None of the permeabilization methods except the freeze‐thaw method enhanced phytase activity. The enzyme activity of a yeast suspension subjected to 15 cycles of freezing and thawing was 77·26 U g−1 cells. Phytate in feeds is an anti‐nutritional factor which complexes with many minerals, rendering it unavailable to monogastric animals. Rhodotorula gracilis subjected to freeze‐thawing can be used as a source of phytase in feeds to enhance phosphorus availability.

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Section: Other Permeabilization Methodsmentioning

confidence: 99%