Effect of Paecilomyces lilacinus on the viability of oncospheres of Taenia hydatigena

Ciarmela, María Laura; Thevenet, Paula Sánchez; Alvarez, Héctor M.; Minvielle, Marta Cecilia; Basualdo, Juan Ángel

doi:10.1016/j.vetpar.2005.04.031

Cited by 13 publications

(6 citation statements)

References 19 publications

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“…Mizobutsi et al (2000), working with 64 isolates of 25 fungal species, among them P. lilacinus , found 77% of parasitism by this species on eggs of Meloidogyne javanica , showing better results than the other species tested. Ciarmela et al (2005) reported a negative biological activity of P. lilacinus on the in vitro viability of Taenia hydatigena oncospheres when compared with the control group.…”

Section: Resultsmentioning

confidence: 99%

Ovicidal activity of Paecilomyces lilacinus on Moniezia sp. eggs

Braga

Araújo

et al. 2008

J. Helminthol.

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The ovicidal activity of Paecilomyces lilacinus was evaluated on Moniezia sp. eggs. Eggs of Moniezia sp. were incubated on plates with 2% agar-water inoculated with grown fungal isolates and a control treatment without fungus. After 5, 10 and 15 days post-inoculation, the eggs were removed and classified according to the following parameters: effect type 1, lytic effect without morphological damage to eggshells; effect type 2, lytic effect with morphological change in embryos and eggshells; and effect type 3, lytic effect with morphological change in embryos and eggshells, with hyphal penetration and internal colonization of eggs. Paecilomyces lilacinus showed percentages for ovicidal activity (P < 0.01), mainly type 3 effect, of 19, 20 and 23% on eggs of Moniezia sp., after 5, 10 and 15 days post-inoculation, respectively. Therefore P. lilacinus can be considered as a potential biological control agent for this cestode.

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Section: Resultsmentioning

confidence: 99%

Ovicidal activity of Paecilomyces lilacinus on Moniezia sp. eggs

Braga

Araújo

et al. 2008

J. Helminthol.

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“…Purpureocillium lilacinum, formerly known as Paecilomyces lilacinus, is a filamentous, soil saprobe fungus that is able to grow in wide pH ranges and uses different substrate sources (LUANGSA-ARD et al, 2011). This species of fungus has played a relevant role in the biological control of various parasites, such as Toxocara canis (BASUALDO et al, 2000;GORTARI et al, 2008;CARVALHO et al, 2010), Taenia hydatigena (CIARMELA et al, 2005), Taenia saginata, Moniezia sp. (BRAGA et al, 2008a,b), Dipylidium caninum (ARAUJO et al, 2009), Oxyuris equi (BRAGA et al, 2012), Fasciola hepatica (NAJAFI et al, 2017) and Ancylostoma (HOFSTÄTTER et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Exposure of Toxocara canis eggs to Purpureocillium lilacinum as a biocontrol strategy: an experimental model evaluation

Filho

Fonseca

Valente

et al. 2019

Rev. Bras. Parasitol. Vet.

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Purpureocillium lilacinum is a nematophagous fungus used in biological control against some parasites, including Toxocara canis. This study researched the infectivity of embryonated T. canis eggs after exposure to the fungus P. lilacinum. T. canis eggs were exposed to P. lilacinum for 15 or 30 days and subsequently administered to Swiss mice (n=20). Control group consisted of mice who received T. canis embryonated eggs without fungal exposure. Forty-eight hours after infection, heart, lung, and liver from animals of each group were collected to assess larval recovery. The organs of mice that received embryonated eggs exposed to the fungus showed a lower average larval recovery (P<0.05) suggesting that exposure of T. canis eggs to P. lilacinum was able to reduce experimental infection. Under the evaluated conditions, the interaction time between the fungus and the parasite eggs was not a significant factor in larvae recovery. P. lilacinum may be considered a promising T. canis biological control agent. However, further studies are needed to determine a protocol for the use of this fungus as a biological control agent.

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“…by Lightowlers et al (1984), Takemoto et al (1995), and Ito et al (1996). Trypan blue has been used for the viability test of exshelled oncospheres (Ciarmela et al 2005;Wang et al 1997), but some limitations have been reported for this vital dye in viability estimates (Chapalamadugu et al 2008). In the present study, an attempt was made to develop an efficient and reproducible method for the in vitro viability test of E. granulosus eggs.…”

Section: Introductionmentioning

confidence: 99%

In vitro viability test for the eggs of Echinococcus granulosus: a rapid method

Moazeni

Rakhshandehroo

2011

Parasitol Res

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In this study an attempt was made to develop an efficient, rapid, simple, and reproducible method for the in vitro viability test of Echinococcus granulosus eggs. The eggs were obtained from an experimentally infected dog and kept at 4°C until use. To prepare the dead or damaged eggs, the eggs were heated in hot water (69-72°C for 10 min), preserved in 70% ethyl alcohol (16 days) or exposed to direct sunlight (18 h). Sodium hypochlorite (0.5-0.7%) was used for the hatching process, and the hatched oncospheres were stained with 0.1% eosin for the viability test. With 0.5% sodium hypochlorite, the hatching rates for viable eggs and eggs killed or damaged by heat (69°C), 70% ethyl alcohol, and direct sunlight were 96%, 97.5%, 91.5%, and 94.6% respectively and there was no significant difference between the hatching rate for viable and dead or damaged eggs (p> 0.05). After staining with 0.1% eosin, the rates of the viable oncospheres hatched from viable eggs and the eggs killed or damaged by heat (69°C), 70% ethyl alcohol, and direct sunlight were 97.5% 3.6%, 7%, and 10.5%, respectively. The difference between the rates of viable oncospheres hatched from viable and dead or damaged eggs was extremely significant (P < 0.0001). With 0.7% sodium hypochlorite, the hatching rates for viable and dead eggs (killed by 72°C for 10 min) were 99.1% and 99.9%, respectively. In this condition, the rate of viable oncospheres was an average of 98.5% for viable eggs and 0.0% for dead ones. The results of this study showed that hatching of eggs by 0.7% sodium hypochlorite and staining of hatched oncospheres by 0.1% eosin are practical methods for the differentiation of viable and nonviable (dead) eggs of Echinococcus granulosus.

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Effect of Paecilomyces lilacinus on the viability of oncospheres of Taenia hydatigena

Cited by 13 publications

References 19 publications

Ovicidal activity of Paecilomyces lilacinus on Moniezia sp. eggs

Ovicidal activity of Paecilomyces lilacinus on Moniezia sp. eggs

Exposure of Toxocara canis eggs to Purpureocillium lilacinum as a biocontrol strategy: an experimental model evaluation

In vitro viability test for the eggs of Echinococcus granulosus: a rapid method

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