SSR-based assessment of genetic diversity in South American Vitis vinifera varieties

Martínez, Liliana; Cavagnaro, Pablo Federico; Masuelli, Ricardo Williams; Zúñiga, María del Carmén

doi:10.1016/j.plantsci.2005.12.006

Cited by 70 publications

(59 citation statements)

References 32 publications

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“…This value was greater than other H e results already obtained by Martínez et al (2006) (81%), Martín et al (2003) (80.6%) and Lamboy and Alpha (1998) (62.5%).…”

Section: Resultscontrasting

confidence: 60%

“…Martín et al (2003), studying 272 grapevine accessions from the Iberian Peninsula in Portugal and Spain, did not find a separation according to the region of origin. Martínez et al (2006) evaluating the diversity of 'Criollas' grapevines from Peru and Argentina, also did not observe separation of groups according to the country of origin. Nevertheless, Dangl et al (2001) obtained separation of cultivars of V. vinifera from the USDA Germplasm Repository according to their geographic origin.…”

Section: Resultsmentioning

confidence: 74%

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Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers

2013

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-The purpose of this research was to study the genetic diversity and genetic relatedness of 60 genotypes of grapevines derived from the Germplasm Bank of Embrapa Semiárido, Juazeiro, BA, Brazil. Seven previously characterized microsatellite markers were used: VVS2, VVMD5, VVMD7, VVMD27, VVMD3, ssrVrZAG79 and ssrVrZAG62. The expected heterozygosity (H e ) and polymorphic information content (PIC) were calculated, and the cluster analysis were processed to generate a dendrogram using the algorithm UPGMA. The H e ranged from 81.8% to 88.1%, with a mean of 84.8%. The loci VrZAG79 and VVMD7 were the most informative, with a PIC of 87 and 86%, respectively, while VrZAG62 was the least informative, with a PIC value of 80%. Cluster analysis by UPGMA method allowed separation of the genotypes according to their genealogy and identification of possible parentage for the cultivars '

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“…This value was greater than other H e results already obtained by Martínez et al (2006) (81%), Martín et al (2003) (80.6%) and Lamboy and Alpha (1998) (62.5%).…”

Section: Resultscontrasting

confidence: 60%

Section: Resultsmentioning

confidence: 74%

Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers

2013

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“…The Ho levels were found to be in the range of 74.3%À85.5% for the grapes grown in different countries. [15,16,18,19,25] In a previous study, Stajner et al [8] reported that the He varied from 0.594 at locus VChr15a to 0.889 at locus VVMD28, with an average He of 0.775 in 121 grape genotypes. The Ho was in the range from 0.315 (VChr8a) to 0.845 (VVMD32) in their study.…”

Section: Genetic Analysismentioning

confidence: 98%

Genetic characterization of autochthonous grapevine cultivars from Eastern Turkey by simple sequence repeats (SSRs)

Eyduran

Erċışlı

Akın

et al. 2015

Biotechnology & Biotechnological Equipment

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In this research, two well-recognized standard grape cultivars, Cabernet Sauvignon and Merlot, together with eight historical autochthonous grapevine cultivars from Eastern Anatolia in Turkey, were genetically characterized by using 12 pairs of simple sequence repeat (SSR) primers in order to evaluate their genetic diversity and relatedness. All of the used SSR primers produced successful amplifications and revealed DNA polymorphisms, which were subsequently utilized to evaluate the genetic relatedness of the grapevine cultivars. Allele richness was implied by the identification of 69 alleles in 8 autochthonous cultivars with a mean value of 5.75 alleles per locus. The average expected heterozygosity and observed heterozygosity were found to be 0.749 and 0.739, respectively. Taking into account the generated alleles, the highest number was recorded in VVC2C3 and VVS2 loci (nine and eight alleles per locus, respectively), whereas the lowest number was recorded in VrZAG83 (three alleles per locus). Two main clusters were produced by using the unweighted pair-group method with arithmetic mean dendrogram constructed on the basis of the SSR data. Only Cabernet Sauvignon and Merlot cultivars were included in the first cluster. The second cluster involved the rest of the autochthonous cultivars. The results obtained during the study illustrated clearly that SSR markers have verified to be an effective tool for fingerprinting grapevine cultivars and carrying out grapevine biodiversity studies. The obtained data are also meaningful references for grapevine domestication.

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“…The number of alleles ranged from 9 (VRZAG29) to 18 (VRZAG79) with a total of 105 alleles and an average of 11.7 alleles per locus. The average number of alleles per locus was higher than that from Spain (10.9) (Moreno-Sanz et al 2011) and South America (9.67) (Martinez et al 2006) with similar microsatellite markers. Some alleles common in European cultivars were also observed in Chinese grape cultivars, but several new alleles were also found, such as alleles 235, 237 and 241 of primer VRZAG79 (Table 3).…”

Section: Microsatellite Polymorphismmentioning

confidence: 59%

“…The high value of PIC confirmed the (Table 2). Martinez et al (2006) also regarded VRZAG62 as the most informative marker in their studies, not VVMD5. Clearly, these findings improved our knowledge of the genetic diversity of grape in China.…”

Section: Microsatellite Polymorphismmentioning

confidence: 99%

Characterization of grape cultivars from China using microsatellite markers

Guo¹,

Zhang²,

Zhang³

2013

CZECH J GENET PLANT

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Guo D.-L., Zhang Q., Zhang G.-H. (2013): Characterization of grape cultivars from China using microsatellite markers. Czech J. Genet. Plant Breed., 49: 164-170.A total of 32 different grape cultivars including representatives of local Chinese cultivars, some important and widely grown Chinese cultivars and international reference cultivars were genotyped at nine microsatellite loci in order to characterize their genetic diversities. The numbers of alleles detected per locus ranged from 9 to 18 with a total of 105 alleles and an average of 11.7 alleles per locus, while the number of microsatellite genotypes varied between 10 and 23, indicating that there are abundant allele diversities in Chinese grape cultivars. The expected heterozygosity varied between 0.740 and 0.915 and the polymorphism information content ranged from 0.716 to 0.908. According to the results of clustering and Principal Coordinates Analysis, three groups were identified among all these cultivars. The clusters of cultivars showed a clear separation of table grape, wine grape of Vitis vinifera and hybrids between European and American species. This study generated a microsatellite profile database for the cultivars from Chinese local and newly bred grapes.

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SSR-based assessment of genetic diversity in South American Vitis vinifera varieties

Cited by 70 publications

References 32 publications

Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers

Diversity and genetic relatedness among genotypes of Vitis spp. using microsatellite molecular markers

Genetic characterization of autochthonous grapevine cultivars from Eastern Turkey by simple sequence repeats (SSRs)

Characterization of grape cultivars from China using microsatellite markers

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