Characterization of grape cultivars from China using microsatellite markers

Guo, Da‐Long; Zhang, Qian; Zhang, Guohai

doi:10.17221/32/2013-cjgpb

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…Microsatellite markers used to differentiate grapevine specimens can effectively complement ampelographic characteristics and are generally used as an advanced molecular management tool within the Vitis genetic resource collection, such as identification and distinction of genotypes, study of genetic diversity, collection creation, study genealogy and verification. These studies allow identifying synonyms, confirming or refuting the previously assumed relationship with varieties from other countries and regions, clarifying the origin (parental forms) of a variety, and proving genetic similarity between autochthonous varieties [5][6][7][8][9][10][11][12].…”

Section: Much Attention Is ---------------------------------------mentioning

confidence: 99%

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Ganich

Naumova

2020

E3S Web Conf.

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The purpose of the research was to study 8 Georgian and 3 Dagestan technical autochthonous grapevine varieties and identify the most productive and promising ones for high-quality winemaking in the Rostov region. The research was conducted in 2016-2019 at Don ampelographic collection named after Ya.I. Potapenko (Novocherkassk, Russia) according to generally accepted methods and state Standards in viticulture. Don autochthonous varieties Sibirkovyij and Krasnostop zolotovskij were used as controls. The soil - ordinary Chernozem carbonate. All the studied varieties had a high percentage of bud break (from 69.3% in Hotsa tsibil to 81.2% in Mahbor tsibil). The fruiting coefficient of 1.0 or more was observed in 6 varieties. The average weight of a bunch varied from 74 g (Mudzhuretuli) to 459 g (Adreuli shavi). The estimated yield was very high for the varieties – Adreuli shavi and Nakutvneuli, high – Goruli mtsvane, Hotsa tsibil, medium – Latsu kere, Grdzelmtevana, Grubela, Amlakhu, Sibirkovyij, Vernakhi and Krasnostop zolotovskij. Grapevine varieties – Goruli mtsvane, Grdzelmtevana, Grubela, Latsu kere, Hotsa tsibil, Nakutvneuli were selected as prospective due to agronomic traits and high quality of wine. These varieties are also recommended to be used in breeding for creating varieties with high technological properties for high-quality winemaking.

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Section: Much Attention Is ---------------------------------------mentioning

confidence: 99%

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Ganich

Naumova

2020

E3S Web Conf.

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“…Множество работ по молекулярно-генетическому исследованию сортов винограда посвящено изучению именно аборигенного генофонда различных регионов виноградарства в мире [10][11][12][13][14][15][16][17][18]. так, результаты генотипирования по 21 микросателлитному локусу 33 стародавних сортов словении позволили выявить несколько форм-синонимов, и при этом опровергнуть ранее предполагаемое близкое родство некоторых генотипов с сортами винограда из соседних стран [12].…”

Section: O R I G I N a L R E S E A R C Hunclassified

Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов L

Ilnitskaya¹,

Naumova²,

Ganich³

et al. 2019

Magarach. Vinogradstvo I Vinodelie

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История виноградарства на Дону насчитывает несколько веков, местные сорта винограда многообразны и специфичны. Микросателлитные маркеры широко используются для генотипирования сортов и подвоев винограда, при изучении происхождения сортов и анализе их родословной. Целью исследования было изучение выборки редких и малораспространенных автохтонных донских сортов и сравнение их с другими аборигенными донскими генотипами на основе данных ДНК-анализа. В исследования включены 23 стародавних донских сорта. Генотипирование проводили методом микросателлитного профилирования. В исследовании использовали микросателлитные маркеры (SSR), рекомендованные в качестве основного минимального набора для ДНК-паспортизации сортов вида Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 и VrZAG79. По результатам проведенного анализа все изученные образцы показали сорт-специфическую комбинацию аллелей в идентифицированных ДНК-профилях. Количество выявленных аллелей составило в среднем 8 аллелей/локус. Наибольший полиморфизм в исследовании этой группы донских сортов был обнаружен в локусе VVMD5: идентифицировано 10 аллелей на локус, наименьшее - в локусе VrZAG62: 6 аллелей/локус. Основываясь на данных SSR-анализа, степень генетического сходства сортов оценивали с использованием метода UPGMA. Кластерный анализ матрицы генетических дистанций, созданный на основе выявленных значений аллелей в шести микросателлитных локусах исследуемых сортов, определил несколько групп генотипов. Сорт Красностоп золотовский выделился в отдельную ветвь, что указывает на различия между этим генотипом и другими сортами исследуемой выборки. Наивысший уровень генетического сходства наблюдался между следующими парами сортов: Крестовский и Бургундский, Шилохвостый и Мушкетный, Кумшацкий черный и Ефремовский.The history of viticulture on the Don goes back several centuries. Local grapevine varieties are diverse and peculiar. Microsatellite markers are widely used in genotyping grapevine cultivars and rootstocks, in grapevine origin and breeding background analysis. Our study aimed to examine samples of rare and less common autochthonous Don varieties, and compare them with the other aboriginal Don genotypes using DNA data. The study involved 23 traditional Don varieties. The genotyping was done by microsatellite profiling. The study used microsatellite (SSR) markers recommended as the basic minimum set for DNA-certification of the genotypes of Vitis vinifera L.: VVMD5, VVMD7, VVMD27, VVS2, VrZAG62 and VrZAG79. Based on the findings, all the studied samples demonstrated variety-specific combination of alleles in the identified DNA profiles. The number of detected alleles on average was 8 alleles/locus. The greatest polymorphism in the studied group of Don varieties was detected in VVMD5 locus: 10 alleles per locus were identified, the smallest in VrZAG62 locus: 6 alleles/locus. UPGMA method was used to assess the extent of genetic similarity of the varieties based on SSR-genotyping data. Based on determined allele values of the studied varieties, cluster analysis of the genetic distances matrix determined several groups of genotypes. ‘Krasnostop zolotovskiy’ variety stood out as a separate branch, which indicates the difference between this genotype and the other varieties of the studied sampling. The highest level of genetic similarity was observed between the following pairs of varieties: ‘Krestovskiy’ and ‘Burgundskiy’, ‘Shilohvostyi’ and ‘Mushketnyi’, ‘Kumshatskiy chornyi’ and ‘Efremovskiy’.

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“…Olive (Olea europaea L.) [23,25,[48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63] Tomato (Solanum lycopersicum L.) [27,44,[64][65][66][67][68][69][70] Grape (Vitis vinifera L.) [15,19,24,[71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89] Wheat (Triticum spp.) [22,26,[90]…”

Section: Crops Referencesmentioning

confidence: 99%

Critical Aspects on the Use of Microsatellite Markers for Assessing Genetic Identity of Crop Plant Varieties and Authenticity of their Food Derivatives

Palumbo¹,

Barcaccia²

2018

Rediscovery of Landraces as a Resource for the Future

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A total of 90 original articles concerning the varietal characterization and identification by means of SSR analysis of the five most economically relevant crops in Italy (i.e., Olea europaea L., Solanum lycopersicum L., Vitis vinifera L., Triticum spp. and Malus Â domestica Borkh.) have been selected and reviewed. Since the genetic traceability of processed products may result more complex, wine and olive oil have been considered too. Specifically, this chapter deals with three main aspects: (i) the criteria adopted for the selection of the most appropriate number, type, and distribution of SSR marker loci to be employed for varietal genotyping, (ii) the use of genetic statistics and parameters for the evaluation of the discriminant ability and applicability of SSR marker loci, and (iii) how to make different experimental works on the same species that are standardized, reliable, and comparable. What emerges from the studies reviewed here is a lack of wider consensus among the authors regarding the strategy to design and to adopt for genotyping plant varieties with SSR markers. This finding highlights the urgent need to establish a common procedure, especially for characterizing and preserving landraces, and for supporting its rediscovery and valorization locally.

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Characterization of grape cultivars from China using microsatellite markers

Cited by 7 publications

References 20 publications

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Autochthonous Georgian and Dagestan grapevine varieties on the collection in the Rostov region

Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов L

Critical Aspects on the Use of Microsatellite Markers for Assessing Genetic Identity of Crop Plant Varieties and Authenticity of their Food Derivatives

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