Spray Drying of Probiotics: Process Development and Scale-Up

Menshutina, Natalia; Гордиенко, М. Г.; Voinovskiy, Alexei A.; Zbiciński, Ireneusz

doi:10.1080/07373937.2010.483043

Cited by 28 publications

(9 citation statements)

References 11 publications

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“…Both freeze-dying and spray-drying which are currently used to dry probiotic cultures expose the culture to extreme environmental conditions [ 6 ]. Spray drying is however more economic and efficient because of its continuous high production rate behavior, but viability of bacteria is usually affected due to use of extreme heat [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Factors affecting viability of Bifidobacterium bifidum during spray drying

et al. 2015

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BackgroundThere is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections. Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms. The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature. The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder.MethodsThe experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content. The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD). Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying. The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer.ResultsSecond-order polynomials were established to identify the relationship between the responses andthe three variables. Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval. The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%. Under optimum conditions, the maximum survival of 28.38% was achieved while moisture was maintained at 4.05%.ConclusionViable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents.

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Section: Introductionmentioning

confidence: 99%

Factors affecting viability of Bifidobacterium bifidum during spray drying

et al. 2015

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“…For this class of products, the drying process not only has to fulfill the basic requirement to obtain a dry powder but also to preserve sensitive compounds. Menshutina et al (2010) studied spray drying of probiotics and showed that the drying process can be optimized with regard to microorganism inactivation by taking into account the drying kinetics together with the inactivation kinetics. Similarly, Mestry et al (2011) employed the response surface methodology to improve the nutritional quality of a fermented juice of carrot and watermelon powders.…”

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confidence: 99%

Impact of the spray drying conditions and residence time distribution on lysine loss in spray dried infant formula

Schmitz-Schug

Foerst

Kulozik

2013

Dairy Sci. & Technol.

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The essential amino acid lysine plays an important role for the nutritional quality of infant formula. Unfortunately, it is easily damaged during spray drying which is usually used to produce infant formula powders. In this study, we showed that the extent of lysine loss can be controlled by adjusting the air inlet temperature and the air-to-liquid ratio. Depending on the spray drying conditions, 10.4±2.9% lysine loss down to no lysine loss was determined after spray drying in laboratory scale and in pilot scale. A main impact on lysine loss could be attributed to the particle residence time which was shown to be longer for the short-form, pilot-scale spray dryer than for the tall-form, laboratory-scale spray dryer. Median particle residence time was 6 s in the laboratory scale dryer and 17 s in the pilot scale dryer. The air-to-liquid ratio of the spray dryer proved to be a good parameter to compare different types of spray dryers. The extent of lysine loss during storage of the spray dried powders was independent of the extent of lysine loss during spray drying. Lysine loss during storage can be reduced by low storage temperatures and low water contents of the powder. Coupling kinetics of lysine loss that take into account the physical state of lactose, i.e., glassy, rubbery, or crystalline, with the processing conditions and particle residence time during spray drying was shown to be a promising tool to improve the nutritional quality of spray dried infant formula.

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“…This technique is the most common method widely used to encapsulate of probiotic cells in the food industry. In this industry, this method is applied for the protection of microbes [71,72]. In this method, living cells are dissolved in polymeric matrices.…”

Section: Spray Dryingmentioning

confidence: 99%

Biopolymers for Biological Control of Plant Pathogens: Advances in Microencapsulation of Beneficial Microorganisms

et al. 2021

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The use of biofertilizers, including biocontrol agents such as Pseudomonas and Bacillus in agriculture can increase soil characteristics and plant acquisition of nutrients and enhancement the efficiency of manure and mineral fertilizer. Despite the problems that liquid and solid formulations have in maintaining the viability of microbial agents, encapsulation can improve their application with extended shelf-life, and controlled release from formulations. Research into novel formulation methods especially encapsulation techniques has increased in recent years due to the mounting demand for microbial biological control. The application of polymeric materials in agriculture has developed recently as a replacement for traditional materials and considered an improvement in technological processes in the growing of crops. This study aims to overview of types of biopolymers and methods used for encapsulation of living biological control agents, especially microbial organisms.

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Spray Drying of Probiotics: Process Development and Scale-Up

Cited by 28 publications

References 11 publications

Factors affecting viability of Bifidobacterium bifidum during spray drying

Factors affecting viability of Bifidobacterium bifidum during spray drying

Impact of the spray drying conditions and residence time distribution on lysine loss in spray dried infant formula

Biopolymers for Biological Control of Plant Pathogens: Advances in Microencapsulation of Beneficial Microorganisms

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