Factors affecting viability of Bifidobacterium bifidum during spray drying

Shokri, Zahra; Fazeli, Mohammad Reza; Ardjmand, Mehdi; Mousavi, Seyyed Mohammad; Gilani, Kambiz

doi:10.1186/s40199-014-0088-z

Cited by 33 publications

(24 citation statements)

References 28 publications

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“…Una estrategia utilizada para mejorar la supervivencia durante el secado por atomización es la exposición de las células de BAL a condiciones subletales desde la etapa de crecimiento; así de esta forma, los microorganismos pueden adaptarse a las condiciones de estrés, dando como resultado, una mejora en su viabilidad [31], [65], [68], [70], [71], [72].…”

Section: Secado Por Atomizaciónunclassified

“…De las condiciones de operación mencionadas anteriormente, la temperatura de entrada y salida de la cámara de secado son las más estudiadas. El rango de la temperatura de entrada de los artículos revisados estuvo entre 80 y 180 ℃ [25], [70], [73], [74], mientras que los rangos de la temperatura de salida fueron entre 40 y 100 ℃ [51], [71]. El aumento de la temperatura de salida disminuye la viabilidad celular, dado que esta temperatura se encuentra por encima de la temperatura que los microorganismos pueden soportar [16], [24].…”

Section: Secado Por Atomizaciónunclassified

“…En [70], encontraron que las condiciones óptimas de proceso, durante el secado por atomización para Bifidobacterium bifidum PTCC 1644 fueron temperatura del aire de entrada (111.15 ℃), presión de aire (4.5 bar) y concentración de maltodextrina (6 %) (Ver tabla en la sección Anexos). Ellos concluyeron que, la temperatura del aire en la cámara de secado, presenta mayor efecto sobre la humedad residual y la supervivencia de los productos secos de Bifidobacterium bifidum PTCC 1644 obtenidos.…”

Section: Secado Por Atomizaciónunclassified

“…%)[70]. De igual modo, Koc y colaboradores (2010), evaluaron el efecto de la temperatura de entrada (150-180 ℃), la temperatura de salida (60-90 ℃) y la temperatura de alimentación (4-30 ℃) durante el secado por atomización de S. thermophilus y L. bulgaricus.…”

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Secado por atomización de bacterias ácido lácticas: una revisión

2019

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Las bacterias ácido lácticas (BAL) son microorganismos que se utilizan como cultivos iniciadores o probióticos, los cuales son de interés en la industria alimentaria y farmacéutica. La preservación de las BAL por diferentes métodos de secado es un tema que ha cobrado interés debido al aumento en la demanda de estos microorganismos. El secado por atomización es un método eficaz que permite obtener productos a bajo costo, con relación al secado por liofilización el cual es comúnmente utilizado para estos microorganismos. El objetivo de esta revisión sistemática es presentar de forma organizada los artículos científicos que han sido publicados en procesos de secado por atomización empleando bacterias ácido lácticas (BAL). La metodología utilizada en la búsqueda y selección de los artículos brinda una exploración durante el periodo 2009- 2019. Los resultados de los diferentes artículos consultados se compararon estableciendo las similitudes metodológicas, se discuten los principales microorganismos estudiados, así como los agentes protectores empleados para mejorar la viabilidad durante el secado por atomización, las condiciones y escala de los equipos de secado por atomización y finalmente las características de los productos secos obtenidos. La información aquí compilada brinda unabase para ajustar la metodología de trabajo cuando se propongan procesos de secado por atomización para BAL.

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Section: Secado Por Atomizaciónunclassified

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Secado por atomización de bacterias ácido lácticas: una revisión

2019

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“…Furthermore, several other factors add on to concerns regarding low survival during spray drying and low stability during storage for application for microorganisms [95]. Factors that play a big role in survival and stability include drying temperature & duration, particle size, type of species/strain to be dried, culture medium, and whether or not pre-adaptation and/or protectants are added [54,96]. Under the right condition, spray drying of lactic acid bacteria can be conducted to achieve sufficiently high retention of cell activity and viability with even comparable quality to those produced through freezedrying [87].…”

Section: Spray Dryingmentioning

confidence: 99%

Microorganism preservation by convective air-drying—A review

2017

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Newcastle University ePrints -eprint.ncl.ac.uk Tan DT, Poh PE, Chin SK. Microorganism preservation by convective air-drying-A review. AbstractAt present, microorganisms are mainly preserved by freeze drying. There is, however, lack of studies conducted on various cheaper yet promising convective air drying alternatives. Convective air drying has been proven to produce dried culture with comparable cell survival and final moisture content to that of freeze drying. This paper aims to draw an understanding to application and suitability of convective air drying which indludes spray-, oven, heat pump, fluidised bed, conveyor, and rotary drying to preserve microorganisms. The paper concludes that drying near ambient temperature and the addition of dehydration protectants are important to obtain satisfactory drying quality. ambient temperature and mild processing conditions [28]. Such condition is hypothesised to be favourable towards high cell survival of microorganisms [29].Majority of studies and reviews, however, largely revolve around freeze-drying and cryogenic preservation which are both costly [8,15,30,31]. Although convective air drying has been used to preserve many foodstuffs, research on its applicability for bacteria preservation is still limited [32][33][34][35][36]. This paper is aimed to critically review work on preservation of microbes; looking into the factors that will affect the cell viability in convective air drying and to suggest future directions on the use of convective air drying for microbial preservation. Methods for microorganism preservationWhile there are many means to preserve a desired culture of microorganisms, some methods might be more suitable than the other depending on the purpose of preservation. Based on applicability of the techniques, preservation methods can be distinguished into those that are limited to just laboratory scale and those that can be applied in industry, as some pilot techniques could be simple and reliable but are not scalable [15]. The key difference between laboratory and industrial scale is the amount of culture that needs to be salvaged [26]. For laboratory purposes, low cell survival is sufficient whereas large quantity (and therefore high cell survival) is required for industrial usage [15]. Typical laboratory preservation techniques include smearing and storing on agar or gelatine, cell immersion in paraffin oil, and the adsorption-desiccation of bacteria culture on filter-paper or on pre-dried plugs of starch or silica gel [15,37,38].High number of successfully preserved viable cells is particularly important to ensure possible direct inoculation to the process fluid where the bacterial culture is to be utilised [11]. The laboratory preservation methods mentioned above are unsuitable due to the complexity of the process, requirement of additives, and low recovery rate [15]. Preservation methods which are recognised to be practical for industrial use are subcultivation, freezing, and drying [39,40].

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Anaerobic Probiotics: The Key Microbes for Human Health

Enshasy

Malik

Malek

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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Human gastrointestinal microbiota (HGIM) incorporate a large number of microbes from different species. Anaerobic bacteria are the dominant organisms in this microbial consortium and play a crucial role in human health. In addition to their functional role as the main source of many essential metabolites for human health, they are considered as biotherapeutic agents in the regulation of different human metabolites. They are also important in the prevention and in the treatment of different physical and mental diseases. Bifidobacteria are the dominant anaerobic bacteria in HGIM and are widely used in the development of probiotic products for infants, children and adults. To develop bifidobacteria-based bioproducts, therefore, it is necessary to develop a large-scale biomass production platform based on a good understanding of the ideal medium and bioprocessing parameters for their growth and viability. In addition, high cell viability should be maintained during downstream processing and storage of probiotic cell powder or the final formulated product. In this work we review the latest information about the biology, therapeutic activities, cultivation and industrial production of bifidobacteria.

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Factors affecting viability of Bifidobacterium bifidum during spray drying

Cited by 33 publications

References 28 publications

Secado por atomización de bacterias ácido lácticas: una revisión

Secado por atomización de bacterias ácido lácticas: una revisión

Microorganism preservation by convective air-drying—A review

Anaerobic Probiotics: The Key Microbes for Human Health

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