Spontaneously and reversibly forming phospholipid polymer hydrogels as a matrix for cell engineering

Ishihara, Kazuhíko; Oda, Haruka; Konno, Tomohiro

doi:10.1016/j.biomaterials.2019.119628

Cited by 28 publications

(21 citation statements)

References 201 publications

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“…Generally, higher dose of GO nanostructure may be cytotoxic to cell proliferations and viability because bare GO caused increase in intracellular stress, irrevocably leading to cell death. Nonetheless, the present observation of cell compatibility outcome confirmed the optimised GO distributions into SF for favourable wound dressing treatment 55‐57 …”

Section: Resultssupporting

confidence: 79%

Novel fabrication of antibiotic containing multifunctional silk fibroin injectable hydrogel dressing to enhance bactericidal action and wound healing efficiency on burn wound: In vitro and in vivo evaluations

Dong

Mao

Zhao

et al. 2021

International Wound Journal

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The development of biologically active multifunctional hydrogel wound dressings can assist effectively to wound regeneration and also has influenced multiple functions on wound injury. Herein, we designed a carbon‐based composited injectable silk fibroin hydrogel as multifunctional wound dressing to provide effective anti‐bacterial, cell compatibility and in vivo wound closure actions. Importantly, the fabricated injectable hydrogel exhibit sustained drug delivery properties, anti‐oxidant and self‐healing abilities, which confirm that composition of hydrogel is highly beneficial to tissue adhesions and burn wound regeneration ability. Frequently, designed injectable hydrogel can be injected into deep and irregular burn wound sites and would provide rapid self‐healing and protection from infection environment with thoroughly filled wound area. Meanwhile, incorporated carbon nanofillers improve injectable hydrogel strength and also offer high fluid uptake to hydrogel when applied on the wound sites. In vitro MTT cytotoxicity assay on human fibroblast cell lines establish outstanding cytocompatibility of the injectable hydrogel and also have capability to support cell growth and proliferations. In vivo burn wound animal model results demonstrate that the hydrogel dressings predominantly influenced enhanced wound contraction and also promoted greater collagen deposition, granulation tissue thickness and vascularization. This investigation's outcome could open a new pathway to fabricate multifunctional biopolymeric hydrogel for quicker burn wound therapy and effectively prevents microenvironment bacterial infections.

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Section: Resultssupporting

confidence: 79%

Novel fabrication of antibiotic containing multifunctional silk fibroin injectable hydrogel dressing to enhance bactericidal action and wound healing efficiency on burn wound: In vitro and in vivo evaluations

Dong

Mao

Zhao

et al. 2021

International Wound Journal

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“…Poly(MPC ‐co‐ BMA ‐co‐ VPBA) (PMBV) was synthesized using conventional radical polymerization with corresponding monomers. [ 27,28 ] The reaction was carried out in an ethanol solution with AIBN as the initiator for polymerization. All monomers were dissolved in ethanol and the total monomer concentration was 0.50 mol L −1 .…”

Section: Methodsmentioning

confidence: 99%

Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Ishihara

Abe

Fukazawa

et al. 2021

Macromolecular Bioscience

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To provide high‐quality cellular raw materials for cell engineering and pharmaceutical engineering, a polymer substrate is prepared for cell separation focusing on the cell proliferation cycle. There are many types of sugar chains on cell membranes, which function as signaling molecules to control interactions with the exterior of the cell; their abundance changes during the cell‐proliferation cycle. In this study, a phenylboronic acid group, which has affinity for sugar chains, is introduced into a polymer containing a phosphorylcholine group that does not induce cell activation. On the surface of this polymer, human promyelocytic leukemia cells can adhere. The adhesion rate is increased by pretreating the substrate with an alkaline solution. Moreover, cell adhesion is dependent on the sugar additive in the culture medium. Therefore, cell adhesion is governed by reactions between the sugar chain on the cell membrane and the phenylboronic acid groups on the substrate. It is revealed that the adhesion rate changes depending on the expression level of sugar chains related to the cell‐proliferation cycle. Based on this, it may be proposed a cell proliferation cycle‐specific separation process using the polymer substrate based on cell adhesion depending on sugar chain density.

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“…[ 5,6 ] In addition, MPC polymer‐based hydrogels have been employed in biomedical applications, including contact lenses and cell containers. [ 7,8 ]…”

Section: Introductionmentioning

confidence: 99%

Application of Zwitterionic Polymer Hydrogels to Optical Tissue Clearing for 3D Fluorescence Imaging

Kojima

Koda

Nariai

et al. 2021

Macromolecular Bioscience

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Zwitterionic polymers have both anion and cation groups in the side chain and have been used in various biomedical applications because of the unique properties. In this study, zwitterionic polymer hydrogels are applied to optical tissue clearing for 3D fluorescence imaging. Polyacrylamide hydrogels have been employed in Clear Lipid‐exchanged Acrylamide‐hybridized Rigid Imaging/Immunostaining/In situ‐hybridization‐compatible Tissue‐hYdrogel method. Zwitterionic polymer hydrogels are produced using zwitterionic monomers, such as 3‐[(3‐acrylamidopropyl)dimethylammonio]propane‐1‐sulfonate (DAPS) and 2‐methacryloyloxyethyl phosphorylcholine (MPC), and crosslinkers. The hydrogels made from poly(DAPS‐co‐acrylamide) and MPC homopolymers afford the most transparent tumor tissues. However, the tissues cleared using DAPS copolymers‐containing hydrogels became turbid in a refractive index‐matching solution, which are unable to obtain clear 3D fluorescence images. In contrast, the 3D fluorescence imaging is achieved in the MPC polymer‐treated 2‐mm‐thick brain slices after immunostaining. The 3D fluorescence imaging of lung metastasis that is cleared by the MPC hydrogel to demonstrate the possible application to cancer diagnosis is performed. The results indicate the increased potentials of zwitterionic polymer hydrogels, especially MPC polymer hydrogels, in biomedical applications.

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Spontaneously and reversibly forming phospholipid polymer hydrogels as a matrix for cell engineering

Cited by 28 publications

References 201 publications

Novel fabrication of antibiotic containing multifunctional silk fibroin injectable hydrogel dressing to enhance bactericidal action and wound healing efficiency on burn wound: In vitro and in vivo evaluations

Novel fabrication of antibiotic containing multifunctional silk fibroin injectable hydrogel dressing to enhance bactericidal action and wound healing efficiency on burn wound: In vitro and in vivo evaluations

Control of Cell‐Substrate Binding Related to Cell Proliferation Cycle Status Using a Cytocompatible Phospholipid Polymer Bearing Phenylboronic Acid Groups

Application of Zwitterionic Polymer Hydrogels to Optical Tissue Clearing for 3D Fluorescence Imaging

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