Spinning‐disk microscopy: a complementary approach to study epithelial precursor cells in dissected human hair follicles

Dimitrov, Ariane; Deshayes, Nathalie; Genty, Gaïanne; Paris, Maryline

doi:10.1111/exd.13048

Cited by 3 publications

(1 citation statement)

References 10 publications

(12 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…identifying a ‘true’ stem cell population (which may not concretely exist, given context specific plasticity). We instead posit that, at least in the bulge, eHFSCs represent a heterogeneous and dynamic pool (Figs S1 and S2 ), likely varying greatly in three dimensions 7 , 49 , 50 . This cell pool can be recognised histologically by a band of nuclei within the basal layer with clearly limited Ki-67 expression/EdU uptake in the bulge region (Fig S2 ).…”

Section: Discussionmentioning

confidence: 92%

Divergent proliferation patterns of distinct human hair follicle epithelial progenitor niches in situ and their differential responsiveness to prostaglandin D2

Purba

Peake

Farjo³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Human scalp hair follicles (hHF) harbour several epithelial stem (eHFSC) and progenitor cell sub-populations organised into spatially distinct niches. However, the constitutive cell cycle activity of these niches remains to be characterized in situ. Therefore, the current study has studied these characteristics of keratin 15+ (K15), CD200+ or CD34+ cells within anagen VI hHFs by immunohistomorphometry, using Ki-67 and 5-ethynyl-2′-deoxyuridine (EdU). We quantitatively demonstrate in situ the relative cell cycle inactivity of the CD200+/K15+ bulge compared to other non-bulge CD34+ and K15+ progenitor compartments and found that in each recognized eHFSC/progenitor niche, proliferation associates negatively with eHFSC-marker expression. Furthermore, we also show how prostaglandin D2 (PGD2), which is upregulated in balding scalp, differentially impacts on the proliferation of distinct eHFSC populations. Namely, 24 h organ-cultured hHFs treated with PGD2 displayed reduced Ki-67 expression and EdU incorporation in bulge resident K15+ cells, but not in supra/proximal bulb outer root sheath K15+ progenitors. This study emphasises clear differences between the cell cycle behaviour of spatially distinct stem/progenitor cell niches in the hHF, and demonstrates a possible link between PGD2 and perturbed proliferation dynamics in epithelial stem cells.

show abstract

Section: Discussionmentioning

confidence: 92%

Divergent proliferation patterns of distinct human hair follicle epithelial progenitor niches in situ and their differential responsiveness to prostaglandin D2

Purba

Peake

Farjo³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

Characterisation of cell cycle arrest and terminal differentiation in a maximally proliferative human epithelial tissue: Lessons from the human hair follicle matrix

Purba

Brunken

Peake

et al. 2017

European Journal of Cell Biology

View full text Add to dashboard Cite

Human hair follicle (HF) growth and hair shaft formation require terminal differentiation-associated cell cycle arrest of highly proliferative matrix keratinocytes. However, the regulation of this complex event remains unknown. CIP/KIP family member proteins (p21, p27 and p57) regulate cell cycle progression/arrest, endoreplication, differentiation and apoptosis. Since they have not yet been adequately characterized in the human HF, we asked whether and where CIP/KIP proteins localise in the human hair matrix and pre-cortex in relation to cell cycle activity and HF-specific epithelial cell differentiation that is marked by keratin 85 (K85) protein expression. K85 expression coincided with loss or reduction in cell cycle activity markers, including in situ DNA synthesis (EdU incorporation), Ki-67, phospho-histone H3 and cyclins A and B1, affirming a post-mitotic state of pre-cortical HF keratinocytes. Expression of CIP/KIP proteins was found abundantly within the proliferative hair matrix, concomitant with a role in cell cycle checkpoint control. p21, p27 and cyclin E persisted within post-mitotic keratinocytes of the pre-cortex, whereas p57 protein decreased but became nuclear. These data imply a supportive role for CIP/KIP proteins in maintaining proliferative arrest, differentiation and anti-apoptotic pathways, promoting continuous hair bulb growth and hair shaft formation in anagen VI. Moreover, post-mitotic hair matrix regions contained cells with enlarged nuclei, and DNA in situ hybridisation showed cells that were >2N in the pre-cortex. This suggests that CIP/KIP proteins might counterbalance cyclin E to control further rounds of DNA replication in a cell population that has a propensity to become tetraploid. These data shed new light on the in situ-biography of human hair matrix keratinocytes on their path of active cell cycling, arrest and terminal differentiation, and showcase the human HF as an excellent, clinically relevant model system for cell cycle physiology research of human epithelial cells within their natural tissue habitat.

show abstract

Human long-term deregulated circadian rhythm alters regenerative properties of skin and hair precursor cells

Deshayes¹,

Genty²,

Berthelot³

et al. 2018

European Journal of Dermatology

View full text Add to dashboard Cite

Spinning‐disk microscopy: a complementary approach to study epithelial precursor cells in dissected human hair follicles

Cited by 3 publications

References 10 publications

Divergent proliferation patterns of distinct human hair follicle epithelial progenitor niches in situ and their differential responsiveness to prostaglandin D2

Divergent proliferation patterns of distinct human hair follicle epithelial progenitor niches in situ and their differential responsiveness to prostaglandin D2

Characterisation of cell cycle arrest and terminal differentiation in a maximally proliferative human epithelial tissue: Lessons from the human hair follicle matrix

Human long-term deregulated circadian rhythm alters regenerative properties of skin and hair precursor cells

Contact Info

Product

Resources

About