Sphingopyxis rigui sp. nov. and Sphingopyxis wooponensis sp. nov., isolated from wetland freshwater, and emended description of the genus Sphingopyxis

Abstract

Two yellow-pigmented, Gram-reaction-negative strains, designated 01SU5-P T and 03SU3-P T , were isolated from the freshwater of Woopo wetland, Republic of Korea. Both strains were aerobic, non-motile and catalase-negative. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Sphingopyxis, showing the highest level of sequence similarity with respect to Sphingopyxis witflariensis W-50 T (95.4-95.7 %). The two novel isolates shared 99.4 % sequence similarity. DNA-DNA hybridization between the isolates and the type strain of S. witflariensis clearly suggested that strains 01SU5-P T and 03SU3-P T represent two separate novel species in the genus Sphingopyxis. The two strains displayed different fingerprints after PCR analysis using the repetitive primers BOX, ERIC and REP. Several phenotypic characteristics served to differentiate these two isolates from recognized members of the genus Sphingopyxis. The data from the polyphasic study presented here indicated that strains 01SU5-P T and 03SU3-P T should be classified as representing novel species in the genus Sphingopyxis, for which the names Sphingopyxis rigui sp. nov. and Sphingopyxis wooponensis sp. nov., respectively, are proposed. The type strain of Sphingopyxis rigui sp. nov. is 01SU5-P T (5KCTC 23326 T 5JCM 17509 T ) and the type strain of Sphingopyxis wooponensis sp. nov. is

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

“…As referred in Table S8, the significant presence of PAHdegrading bacteria like Comamonas (Goyal and Zylstra 1996) and Diaphorobacter (Klankeo et al 2009) indicated the removal of corresponding pollutants in aerobic sludge leading to detoxification of CWW (Zhang et al 2013). The high abundances of NHC-degrading bacteria Brevundimonas, Sphingopyxis, Sphingosinicella Stolz 2009;Baik et al 2013), and Comamonas (Felföldi et al 2010;Zhang et al 2015) also tallied with the complete disappearance of NHCs in the final effluent in this study. The NHCutilizing bacteria detected in CWW treating sludge exhibit considerable potential for biotechnological applications.…”

Massilia chloroacetimidivorans sp. nov., a chloroacetamide herbicide-degrading bacterium isolated from soil

“…This threshold has been reasonably increased to between 98.2 and 99.0% depending on the taxonomic group which is to be investigated (Meier-Kolthoff et al 2013;Stackebrandt and Ebers 2006). Therefore, DNA-DNA hybridisation experiments were performed between strain TA-C7e T and three closely related species of the genus Massilia according to the methods of Baik et al (2013). DNA-DNA hybridisation was carried out by the membrane filter technique using the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche Applied Science).…”

Sphingorhabdus lacus sp. nov. and Sphingorhabdus profundilacus sp. nov., isolated from freshwater environments

“…Strains IMCC1753 T and IMCC26285 T contained PE, PME, PC, PG, DPG and SGL as major polar lipids (Fig. S3), similar to major polar lipids of Sphingorhabdus [9,12,[15][16][17]. The major polyamine and polar lipids of both strains were highly similar except for the presence of an unidentified glycolipid and an unidentified lipid in strain IMCC26285 T .…”

“…Polar lipids were extracted according to the procedures described by Minnikin [17]. †Data from Baik et al [16]. ‡Data from Jogler et al [9].…”

“…The genus Sphingorhabdus of the family Sphingomonadaceae was first proposed by Jogler et al [9] with the description of Sphingorhabdus planktonica and the reclassification of three Sphingopyxis species. Recently, four species of the genus Sphingorhabdus have been transferred to the novel genus Parasphingorhabdus [10], and therefore the genus Sphingorhabdus currently comprises nine species with validly published names which have been isolated from diverse environments such as freshwater [11,12], seashore sediment [13], arctic soil [14], seawater [15], wetland [16] and contaminated Petri dish [17]. The genus is characterized by Gram-stain-negative and aerobic bacteria that contain ubiquinone-10 (Q-10) as the major respiratory quinone, spermidine as the major polyamine and phosphatidylethanolamine (PE), phosphatidylmethylamine (PME), phosphatidylcholine (PC), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and SGL as the major polar lipids [9,12,14].…”