Sphingobacterium shayense sp. nov., isolated from forest soil

Abstract

A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39 T , isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30-37 6C, at pH 6.5-8.0 and with 0-3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39T revealed that it is a member of the genus Sphingobacterium.Sphingobacterium mizutaii ATCC 33299 T was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 (comprising C 16 : 1 v6c and/or C 16 : 1 v7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39 T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39 T (5CCTCC AB 209006 T 5NRRL B-59203 T ).

Sphingobacterium cladoniae sp. nov., isolated from lichen, Cladonia sp., and emended description of Sphingobacterium siyangense

“…They contain menaquinone 7 (MK-7) as the predominant isoprenoid quinone, and iso-C 15 : 0 , iso-C 15 : 0 2-OH, C 16 : 1 v7c and C 17 : 0 3-OH as the main fatty acids. The range of DNA G+C content is approximately 35-44 mol% (Liu et al, 2008;He et al, 2010).…”

Sphingobacterium lactis sp. nov. and Sphingobacterium alimentarium sp. nov., isolated from raw milk and a dairy environment

“…At the time of writing, the genus encompassed the following 15 species with validly published names: Sphingobacterium multivorum, S. mizutaii and the type species S. spiritivorum (Yabuuchi et al, 1983), S. antarcticum (Shivaji et al, 1992), S. faecium and S. thalpophilum (Takeuchi & Yokota, 1992), S. daejeonense (Kim et al, 2006), S. composti T5-12 T , S. composti 4M24 T (Yoo et al, 2007), S. canadense (Mehnaz et al, 2007), S. siyangense (Liu et al, 2008), S. kitahiroshimense (Matsuyama et al, 2008), S. anhuiense (Wei et al, 2008), S. shayense (He et al, 2010) and S. bambusae (Duan et al, 2009). There are two validly named S. composti strains, described by Ten et al (2006) and by Yoo et al (2007), respectively.…”

“…Hydrolysis of: 2006; Mehnaz et al, 2007;Yoo et al, 2007;Liu et al, 2008;Matsuyama et al, 2008;Wei et al, 2008;Duan et al, 2009;He et al, 2010). Respiratory quinones were analysed according to Altenburger et al (1996).…”

Sphingobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside-converting activity isolated from the soil of a ginseng field

“…T (Marqués et al, 2012), S. shayense (He et al, 2010), S. siyangense (Liu et al, 2008), S. kitahiroshimense (Matsuyama et al, 2008), S. canadense (Mehnaz et al, 2007), S. anhuiense (Wei et al, 2008), and S. composti (Yoo et al, 2007). It is the type genus of the family Sphingobacteriaceae (Steyn et al, 1998) in the phylum Bacteroidetes and currently consists of 21 species with validly published names, with S. multivorum as the type species (Euzéby, 1997).…”