Sperm Sexing in Animals

Sharma, Mridula; Sharma, Nishant

doi:10.14737/journal.aavs/2016/4.10.543.549

Cited by 12 publications

(9 citation statements)

References 38 publications

(46 reference statements)

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“…Sex sorting of sperms is reproductive biotechnology which capable to control the sex of calves born for specific purposes by separating sperm-bearing X-and Y-chromosomes. Sperm with Xand Y-chromosome have several characteristic differences such as DNA content, morphology, motility, mass weight, and specific genes contained therein (Sharma and Sharma, 2016). The current research focused on the qualitative differences of spermatozoa bearing X-and Y-chromosomes separated by using albumin gradient and swim-up methods in local Pesisir cattle in West Sumatra, Indonesia.…”

Section: Journal Of Animalmentioning

confidence: 99%

Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle

Afriani¹,

Udin²,

Hellyward³

et al. 2022

JAHP

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Section: Journal Of Animalmentioning

confidence: 99%

Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle

Afriani¹,

Udin²,

Hellyward³

et al. 2022

JAHP

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“…resis, filtration by Sephadex column, HY antigen, and flowcytometry sorting cells (Sharma & Sharma, 2016). However, due to various advantages and disadvantages, only a few of these are commonly used for sexing.…”

Section: Successful Separation Of X-and Y-spermatozoa Ongole Crossbre...mentioning

confidence: 99%

Successful Separation of X- and Y-Spermatozoa Ongole Crossbreed Using a Nano-Albumen Gradient Column

Maulana

Ridwan

Gunawan

et al. 2022

Trop. Anim. Sci. J.

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The objective of this study was to determine the efficacy of separating X-and Y-sperm using nano-albumen (NA). The material used in this study was fresh semen of Ongole Crossbreed bulls with progressive motility of ≥ 70%. Treatments for the top and bottom fractions were T0 (BSA 5% : 10%) as a control, T1 (NA 2% : 4%), T2 (NA 4% : 6%), and T3 (NA 6% : 8%). Sexed semen was evaluated for characteristics and kinematics, and the proportions of sexed semen were validated using morphometric and polymerase chain reaction methods. The data on the characteristics and motility kinematics of X-and Y-sperm were analyzed by ANOVA using Minitab 18. The results revealed that the semen separated by the NA column generally showed the same sperm quality characteristics as the BSA column. However, the total motility of the T3y treatment in the bottom fraction was significantly lower (p<0.05) compared to the control. The kinematics of spermatozoa motility in the top fraction was not significantly different; however, the progressive motility value of T3y was significantly different (p<0.05) compared to that of T1y, and the BCF value of T2y was significantly different from that of the control in the bottom fraction. The acrosome status in the top fraction was better than in the bottom fraction, and the DNA integrity value of the top and bottom fractions showed DNA damage ranging from 2% to 5%. The present study indicated that: (1) the Nano-Albumen gradient column could be used successfully to separate X-and Y-spermatozoa, (2) characteristic values and sperm kinematics, acrosomal status, and DNA integrity were comparable to those of the BSA control, (3) combination column in treatments NA 4% (T2x) and 6% (T2y) appeared the good ability to separated X and Y-sperm.

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“…Sexing technology is the process of separating X and Y spermatozoa, which is one technology to obtain the desired calf birth [11]. Various techniques and methods of sperm sexing have been applied to various farm animals [12]; [13]; [14] [15]; [16]. The techniques used to separate X and Y spermatozoa, one of which is Egg White Freeze Dry or commonly called freeze dry egg whites, Freeze drying method is based on being able to produce products with relatively higher quality than with another drying.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time

Damayanti

Yusuf²,

Ako³

et al. 2022

HAJAS

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The development of reproductive technology for the separation of spermatozoa with X and Y chromosomes, which is better known as sexing, can be carried out in vitro using various methods, one of which is using freeze dry egg whites. This study aims to determine the quality of semen based on incubation time. This study used four types of treatment, namely P0 = No Incubation, P1 = Incubation 30 minutes, P2 = Incubation 45 minutes, P3 = Incubation 60 minutes with six replications. Parameters observed were the quality of spermatozoa macroscopically (volume, odor, color and consistency) and microscopically (motility, abnormality, concentration, intact plasma membrane, intact acrosome cap). Y using descriptive statistical test. The results of analysis of variance that sexing spermatozoa using freeze dry egg whites at different incubation times showed a significant effect (P<0.05) on intact plasma membrane (IPM), intact acrosomal membrane (IAM), but had no significant effect P>0.05) on concentration, motility, and abnormalities. Evaluation of fresh Bali cattle spermatozoa showed results that were in accordance with the standards for further examination of semen sexing. Incubation time of 30 minutes in the sexing process of spermatozoa caused a significant decrease in motility and concentration, meanwhile, IPM and IAM of sexed spermatozoa experienced a significant decrease in incubation time of 60 minutes, but the decrease was still within the normal range for spermatozoa. to be used in the artificial insemination process. Keywords: Sexing, incubation, concentration, motility, abnormality, intact plasma membrane, intact acrosome caps.

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Sperm Sexing in Animals

Cited by 12 publications

References 38 publications

Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle

Separation of Bull Spermatozoa Bearing X- and Y-Chromosome by using Albumin Gradient and Swim-up Technique in Pesisir Cattle

Successful Separation of X- and Y-Spermatozoa Ongole Crossbreed Using a Nano-Albumen Gradient Column

Characteristics of Bali Bull (Bos Sondaicus) Sexed Sperms with Freeze Dry Egg White at Different Incubation Time

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