Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species

Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species

“…Cell viability after thawing was higher in the vitrification group (95%) and rapid freezing group (72%) as compared to the slow freezing group (24%). In another study, conducted by BAERT et al (2012), testicular tissue fragments of These results are mostly consistent with those reported by THUWANUT et al (2013). They assessed the effect of slow and rapid freezing on the testicular tissue of wild cats (Felis chaus; Panthera leo; Panthera pardus), and reported that testicular tissue subjected to rapid freezing had better plasma membrane integrity.…”

“…The fragments are then kept in nitrogen vapor at 4-5cm above the liquid column for 10 minutes, after which they are allocated to cryotubes and dipped in liquid nitrogen (THUWANUT et al, 2013). Avoiding cryoinjuries during the cryopreservation steps is the biggest challenge faced when using this technique (BUARPUNG et al, 2013).…”

“…Spermatogenic cells present in cryopreserved testicular fragments can resume their functions in vitro after the testicular fragments have been thawed, contributing to the preservation of fertility and genetic resources of animals considered to be of high genetic value (PUKAZHENTHI et al, 2015), as well as the preservation of those considered endangered wild species (THUWANUT et al, 2013).…”

“…This remains a major challenge in the preservation of fertile animals with superior genetics, preservation of human reproductive health, as well as in the establishment of cryobanks for wildlife (HOLOCH & WALD, 2011;UNNI et al, 2012;THUWANUT et al, 2013;BAERT et al, 2015).…”

Evaluation of different fragment sizes and cryoprotectants for cryopreservation of feline testicular tissues

“…In veterinary medicine, this technique has been shown to be a reliable and innovative alternative to conserve male genetic material of any animal that has to be subjected to therapeutic orchiectomy or that died unexpectedly (Buarpung, Tharasanit, Comizzoli, & Techakumphu, ). Moreover, reports have shown that the sperm cells obtained after the freezing–thawing process and submitted to testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) resulted in the development of embryos and the birth of viable offsprings (Abrishami, Anzar, Yang, & Honaramooz, ; Bono‐Mestre, Cardona‐Costa, & García‐Ximénez, ; Buarpung, Tharasanit, Comizzoli, & Techakumphu, ; Hori, Ichikawa, Kawakami, & Tsutsui, ; Hori, Matsuda, Kobayashi, Kawakami, & Tsutsui, ; Jahnukainen, Ehmcke, Hergenrother, & Schlatt, ; Marks, Dupuis, Mickelsen, Memon, & Platz, ; Milazzo et al., , ; Schlatt, Foppiani, Rolf, Weinbauer, & Nieschlag, ; Shinohara et al., ; Thuwanut et al., ; Wu et al., ).…”