Evaluation of different fragment sizes and cryoprotectants for cryopreservation of feline testicular tissues

Macente, Beatrice Ingrid; Toniollo, Gilson Hélio; Apparício, M.; Mansano, Cleber Fernando Menegasso; Thomé, Helder Esteves; Canella, C. L.; Tozato, Maria Eduarda Gonçalves; Gutiérrez, Rodolfo Gozalo

doi:10.1111/rda.12828

Cited by 10 publications

(3 citation statements)

References 25 publications

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“…9 Testes contain a large number of germ cells, especially spermatogonia that can provide an unlimited source of male gametes if appropriately preserved and cultured. 10 In domestic species and also in some wild mammals, several studies have shown collection and preservation of testes from sexually immature individuals 11,12 and adult animals, 13 alive 14 or postmortem. 15 In general, a first strategy consists of conserving testicular tissue fragments appropriately using cryopreservation systems, such as slow freezing, fast freezing, and vitrification.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Silva

Pereira

Comizzoli

2020

Biopreservation and Biobanking

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Systematic cryo-banking of reproductive tissues could enhance reproductive management and ensure sustainability of rare mammalian genotypes. Testicular tissues contain a vast number of germ cells, including at early stages (spermatogonia and spermatocytes), that can potentially develop into viable spermatozoa after grafting or culture in vitro, and the resulting sperm cells then can be used for assisted reproductive techniques. The objective of this review was to describe current advances, limitations, and perspectives related to the use of testicular tissue preservation as a strategy for the conservation of male fertility. Testes can be obtained from mature or prepubertal individuals, immediately postmortem or by orchiectomy, but testicular biopsies could also be an alternative to collect samples from living individuals. Testicular fragments can be then cryopreserved by using slow or ultra-rapid freezing, or even vitrification methods. The composition of cryopreservation media can vary according to species-specific characteristics, especially regarding the cryoprotectant type and concentration. Finally, spermatozoa have been usually obtained after xenografting of testicular fragments into severely immunodeficient mice, while this method still has to be optimized after in vitro culture conditions.

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Section: Introductionmentioning

confidence: 99%

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Silva

Pereira

Comizzoli

2020

Biopreservation and Biobanking

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“…Fragments with 0.5 cm 3 of volume were compared to 0.3 cm 3 fragments. Larger fragments (0.5 cm 3 ) had less harmful effects on germ cells than smaller ones (0.3 cm 3 ) [ 23 ].…”

Section: Feline Speciesmentioning

confidence: 99%

“…The use of different cryoprotectants such as glycerol and propanediol has been proposed in the rapid freezing of cat testis. However, the histomorphological characteristics are better-preserved using glycerol [ 23 ].…”

Section: Feline Speciesmentioning

confidence: 99%

Canine and Feline Testicular Preservation

Silva

2022

Animals

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The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.

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Recent Advances in Fertility Preservation and Genome Resource Banking for Rare and Endangered Animal Species

Comizzoli,

Holt

2024

Cryopreservation in Assisted Reproduction

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Evaluation of different fragment sizes and cryoprotectants for cryopreservation of feline testicular tissues

Cited by 10 publications

References 25 publications

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation

Canine and Feline Testicular Preservation

Recent Advances in Fertility Preservation and Genome Resource Banking for Rare and Endangered Animal Species

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