Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species

Lima, David Baruc Cruvinel; Silva, Lúcia Daniel Machado da

doi:10.1590/0103-8478cr20170135

Cited by 6 publications

(8 citation statements)

References 28 publications

(56 reference statements)

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“…Spermatogenesis is a complex process requiring adequate germ cell environment that may be impaired after warming and reanimation [ 18 , 19 ]. Production of spermatozoa from frozen-thawed testicular tissue (followed by the birth of healthy offspring after oocyte fertilization and embryo transfer) is possible in the mouse model [ 11 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model

2018

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Understanding critical roles of warming and reanimation is critical to improve the survival of vitrified testicular tissue in domestic cats. The objective was to study structural and functional properties of testicular tissues from prepubertal domestic cats after standard vitrification followed by two warming protocols (directly at 37°C or with a 5-second pre-exposure to 50°C) and three reanimation time points (immediately, 24 h and 5 days post-warming). In Experiment 1, tissues were evaluated for histo-morphology and mitochondrial activity immediately or 24 h after warming protocols. In Experiment 2, cell viability, DNA fragmentation, and germ cell composition were assessed immediately, 24 h, or 5 days after optimal warming. Preservation of seminiferous tubule structure was better using warming at 50°C for five seconds, and survival of somatic as well as germinal cells was higher compared to direct warming at 37°C for one minute. Short term in vitro culture (for reanimation) also proved that cellular composition and functionality were better preserved when warmed for a short time at 50°C. Collective data showed that short warming at 50°C led to better quality of seminiferous tubule structure and cell composition after vitrification and short-term culture. In addition, data suggest clear directions to further understand and optimize testicular tissue survival after fertility preservation procedures.

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Section: Introductionmentioning

confidence: 99%

Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model

2018

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“…The remaining fragments were used for vitrification by needle immersion (Wang et al., 2008). The association between cryoprotectant dimethyl sulphoxide and glycerol was used (Lima and Silva, 2017). The fragments were placed in 30G hypodermic needles and initially immersed in an equilibrium solution containing 1.4 M of each cryoprotectant and 0.25 M of sucrose and minimum essential medium (MEM–Ham's F10) for 10 min at room temperature (~ 22°C).…”

Section: Methodsmentioning

confidence: 99%

“…(Moreira, 2017). Obtaining the biological material is important in adverse situations as early death, especially in the collection of genetic material of endangered species (Comizzoli, 2017; Thuwanut et al., 2013) or those that will be previously exposed to gonadotoxic factors (Oliveira, 2015; Lima and Silva, 2017), whether for animal models (Tanpradit et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The testicular cryopreservation is a technique that can promote the preservation of the reproductive potential of domestic and wild pre‐pubertal felids (Comizzoli, 2017; Oliveira, 2015). Due to the impossibility of obtaining the gametes in the pre‐pubertal phase, the testicular cryopreservation is the only alternative to preserve the future fertility, which can be obtained through in vitro or in vivo culture of the testicular fragments (Pukazhenthi et al., 2015; Lima and Silva, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Influence of different warming temperatures on the vitrified testicular fragments from pre‐pubertal cat

Fernandes

Tabosa

Brito

et al. 2021

Reprod Domestic Animals

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Testicular vitrification is an alternative to preserve the genetic material of pre‐pubertal animals. However, there are few studies on post‐vitrification warming. Hence, the aim was to compare the influence of different warming temperatures on vitrified testicular fragments from pre‐pubertal cats. The testicles were fragmented and divided into a control group (non‐vitrified) and vitrified, using an association between dimethylsulphoxide and glycerol. The vitrified fragments were warmed at 50, 55 and 60°C/5 s. Morphological and morphometric evaluations were carried out using classical histology. Afterwards, the mitochondrial activity was evaluated using Rhodamine 123. The data were expressed in mean and standard error. The differences were considered significant when p < .05. In the histomorphological analysis, the testicular fragment presented seminiferous tubules with poorly developed germinal epithelium, compatible with pre‐pubertal animals. The group warmed at 50°C presented similar to the control regarding the maintenance of the integrity of the tubules and cells, without stromal rupture and lamina propria alteration, as well as regarding the maintenance of the junctions between the cells. The group warmed at 55°C showed reduction of the cell junctions, and the one warmed at 60°C had increased detachment of the basement membrane (p < .05). The warming caused a reduction in the tubular diameter inversely proportional and progressive to the increase in temperature, with the highest diameter in the control group and the lowest in the 60°C group. The control group showed a lower incidence of Rhodamine 123, followed in ascending order of the warmings at 55 and 60°C. The higher mitochondrial activity was obtained with 50°C, showing an increase of the metabolic cell function at this temperature. It was concluded that the testicular fragment of pre‐pubertal cats presents a better preserved morphology, morphometry and viability when warmed at 50°C.

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“…Testicular cryopreservation enables the maintenance of reproductive capacity [ 1 , 2 , 3 , 4 ] and allows the implantation of germplasm banks for several species of commercial value or even those at risk of extinction [ 5 ]. Moreover, it provides the transport of genetic material among different regions [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Canine and Feline Testicular Preservation

Silva

2022

Animals

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The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.

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Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species

Cited by 6 publications

References 28 publications

Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model

Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model

Influence of different warming temperatures on the vitrified testicular fragments from pre‐pubertal cat

Canine and Feline Testicular Preservation

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