Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls

Kusumawati, Asmarani; Satrio, Faisal Amri; Indriastuti, Rhesti; Rosyada, Zulfi Nur Amrina; Pardede, Berlin Pandapotan; Agil, Muhammad; Purwantara, Bambang

doi:10.3390/ani13152433

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…In contrast, there was no substantial difference in sperm head defects between the age groups (P>0.05). Chenoweth (2005) , Carreira et al (2017) , and Kusumawati et al (2023) reported that sperm head defects, which are significant abnormalities in sperm, are generally caused by various things, such as genetic factors and DNA condensation. High sperm head defects were also reported to correlate with decreased bull fertility ( Lemma, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Bos sondaicus)

Fatmila,

Pardede,

Maulana

et al. 2024

Anim. Reprod.

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This study aimed to analyze the characteristics of the HSP70 gene and protein in spermatozoa of Bali bulls of different age groups and to examine its potential as a biomarker determining bull fertility. This study used frozen semen produced from six Bali bulls divided into two groups based on age (≤ 9 years and ≥ 12 years). Parameters of frozen semen quality analyzed included sperm motility and kinetics using computer-assisted semen analysis, sperm morphological defects using Diff-Quick staining, acrosome integrity using FITC-PNA staining, and DNA fragmentation using acridine orange staining. HSP70 gene expression characterization was analyzed using qRT-PCR, and HSP70 protein abundance was analyzed using enzyme immunoassays. Fertility field data were obtained by analyzing the percentage conception rate for each bull based on the artificial insemination service data contained in the Indonesian-integrated system of the National Animal Health Information System (iSIKHNAS). The results showed significant differences (P<0.05) in total and progressive motility, morphological defects of the neck and midpiece, and tail of sperm, and acrosome integrity between the age groups of Bali bulls. HSP70 gene expression and protein abundance showed no significant differences (P>0.05) in different age groups. HSP70 gene expression correlated with fertility rate (P<0.05). Age affected several semen quality parameters but did not affect HSP70 gene expression and protein abundance. The HSP70 gene molecule could be a biomarker that determines the fertility of Bali bulls.

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Section: Discussionmentioning

confidence: 99%

Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Bos sondaicus)

Fatmila,

Pardede,

Maulana

et al. 2024

Anim. Reprod.

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Bovine nucleoprotein transitions genes and protein abundance as valuable markers of sperm motility and the correlation with fertility

Pardede,

Karja,

Said

et al. 2024

Theriogenology

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Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull

Satrio,

Karja,

Setiadi

et al. 2024

Front. Vet. Sci.

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Increasing the age of bulls results in a decrease in reproductive function, including a reduction in sperm quality, which plays a vital role in determining the fertility of bulls. Through a proteomic approach, this research aims to analyze the influence of age factors on various proteomes contained in bull sperm. Frozen semen samples from Simmental Bulls were categorized into three age groups: two, four, and ≥10 years old. Subsequently, the post-thaw sperm cells obtained were separated based on molecular weight using 1D-SDS-PAGE. Peptides extracted from the bands produced in each age group were subjected to LC-MS/MS analysis. A total of 72 protein types were identified, with 45 being detected in the 4-year-old group and 41 expressed in both the 2 and ≥10-year-old groups. The results provided insights into proteins' role in sperm metabolism across all age groups. Specifically, the 2-year-old group exhibited the expression of proteins associated with acrosome assembly and spermatid development (SPACA1). In contrast, those in the 4-year-old group were linked to motility (PEBP4) and sperm decapacitation factor (PEBP1). Proteins expressed in the 2 and -year-old groups were discovered to be involved in fertilization processes (TEX101). In contrast, the ≥10-year-old age group was associated with hyperactive movement related to capacitation (Tubulin). In conclusion, age influenced the differences observed in the proteomic profile of post-thaw Simmental bull sperm using the 1D-SDS-PAGE tandem LC-MS/MS approach.

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Sperm Head Morphology Alterations Associated with Chromatin Instability and Lack of Protamine Abundance in Frozen-Thawed Sperm of Indonesian Local Bulls

Cited by 3 publications

References 30 publications

Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Bos sondaicus)

Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls (Bos sondaicus)

Bovine nucleoprotein transitions genes and protein abundance as valuable markers of sperm motility and the correlation with fertility

Age-dependent variations in proteomic characteristics of spermatozoa in Simmental bull

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