Sperm concentration, hyaluronic acid-binding capacity, aneuploidy and persistent histones in testicular cancer

Molnár, Zsolt; Mokánszki, Attila; Kassai, Behrouz; Bhattoa, Harjit Pal; Benyó, Mátyás; Oláh, Éva; Jakab, Attila

doi:10.1093/humrep/deu179

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…The present study shows a significantly elevated rate of sperm aneuploidy at T0 in accordance with the locoregional or systemic toxicity of the cancer itself able to perturb the meiotic process, resulting in diminished sperm production and increased number of aneuploid spermatozoa (4,18,19). Our data do not agree with the recent report that concludes that semen samples dedicated for cryopreservation before TC treatment initiation do not carry an elevated risk for numeric chromosome aberrations (27). In addition, our study confirms a significantly higher frequency of sperm chromosome abnormalities at T6 (18,19), due to chromosome nondisjunctions occurring during the two meiotic divisions.…”

Section: Chromosome Abnormality Before Treatmentcontrasting

confidence: 94%

Sperm aneuploidy after testicular cancer treatment: data from a prospective multicenter study performed within the French Centre d’Étude et de Conservation des Oeufs et du Sperme network

Rives

Walschaerts

Sétif

et al. 2017

Fertility and Sterility

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Genetic counseling of TC patients should include information on the potential elevated risk of aneuploid conceptus from sperm recovered after treatment and the necessity to postpone conception up to ≥12 months after radiotherapy and ≥24 months after more than two BEP chemotherapy cycles. However, few men receiving one or two BEP cycles and some dropouts are the main limitations of this study.

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Section: Chromosome Abnormality Before Treatmentcontrasting

confidence: 94%

Sperm aneuploidy after testicular cancer treatment: data from a prospective multicenter study performed within the French Centre d’Étude et de Conservation des Oeufs et du Sperme network

Rives

Walschaerts

Sétif

et al. 2017

Fertility and Sterility

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“…Molnar et al (2014) note that, in samples taken from a group of 28 postorchiectomy TGCT patients, 56.9% of sperm cells were HA-bound (mean value). In addition, a decreased HA-binding capacity and a higher aneuploidy frequency were observed [38]. It can be assumed that sperm maturation is significantly impaired in the cancer patients, probably as a result of the process leading to the formation of neoplasia.…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA Fragmentation Index and Hyaluronan Binding Ability in Men from Infertile Couples and Men with Testicular Germ Cell Tumor

Marchlewska

Filipiak

Walczak-Jędrzejowska

et al. 2016

BioMed Research International

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Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT). Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (DFI) and the ability of sperm to bind with hyaluronan (HA) was assessed. Results. In comparison with the IC men, the men with TGCT had a higher percentage of sperm with fragmented DNA (median 28% versus 21%; p < 0.01) and a lower percentage of HA-bound sperm (24% versus 66%; p < 0.001). Normal results of both analyses were observed in 24% of IC men and 4% of men with TGCT. Negative Spearman's correlations were found between DFI and the percentage of HA-bound sperm in the whole group and in IC subjects and those with TGCT analyzed separately. Conclusions. Approximately 76% of IC men and 96% with TGCT awaiting orchiectomy demonstrated DNA fragmentation and/or sperm immaturity. We therefore recommend sperm banking after unilateral orchiectomy, but before irradiation and chemotherapy; the use of such a deposit appears to be a better strategy to obtain functionally efficient sperms.

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“…For HBA two types of 'responders' were defined: (i) 'responders A' with an improvement above the double of the IV value; (ii) 'responders B' with a % HA binding greater than an absolute HBA value equal or above 60% (compatible with lower limit value for normal semen according to Huszar et al, 2002;Kovacs et al, 2011;Molnar et al, 2014).…”

Section: Definition Of Responders For Tsn Tmsc and Hba Valuesmentioning

confidence: 99%

Short‐term FSH treatment and sperm maturation: a prospective study in idiopathic infertile men

et al. 2017

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The standard FSH treatment is based on a 3 months period, after which both quantitative/qualitative improvement of sperm parameters and increased pregnancy rate were reported. In this prospective clinical trial, for the first time, we studied (i) Sperm hyaluronic acid binding capacity after highly purified FSH (hpFSH) treatment; (ii) the effect after short-term and standard treatment on this functional parameter. As secondary objective, we analyzed three SNPs on FSHβ and FSHR genes to define their potential predictive value for responsiveness. From a total of 210 consecutive patients, 40 oligo- and/or astheno- and/or teratozoospermic patients fulfilled the inclusion criteria. Treatment consisted in hpFSH 75 IU/L every other day for 3 months. To avoid potential biases derived from the lack of placebo, we analyzed each patient after 4-6 months of 'wash-out' period. After FSH treatment, we observed a statistically significant (p < 0.001) improvement of the percentage of hyaluronic acid bound spermatozoa from basal to T1 (after 1 month) and to T3 (after 3 months). Importantly, these values returned to near-baseline value after the wash-out. The same results were detected for total motile sperm count after 3 months with return to baseline after wash-out. Forty-two percent of patients responded to the therapy with increasing hyaluronic acid binding capacity above the double of the Intraindividual Variation (IV) while 24% of patients reached above the normal Sperm-Hyaluronan Binding Assay (HBA) value. Further increase in 'responders' was observed at T3. The responsiveness to treatment resulted independent from FSHR/FSHβ polymorphisms. The significant positive effect on sperm maturity after 1 month opens novel therapeutic perspectives. In view of both the high cost and the relative invasiveness of treatment, the short protocol (1 month) could represent a viable FSH treatment option prior Assisted Reproductive Techniques since FSH, by acting on sperm maturation, increases the proportion of functionally competent cells.

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Sperm concentration, hyaluronic acid-binding capacity, aneuploidy and persistent histones in testicular cancer

Abstract: This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP-4.2.4.A/2-11/1-2012-0001 'National Excellence Program'. The authors have no conflict of interest to declare.

Cited by 8 publications

References 51 publications

Sperm aneuploidy after testicular cancer treatment: data from a prospective multicenter study performed within the French Centre d’Étude et de Conservation des Oeufs et du Sperme network

Sperm aneuploidy after testicular cancer treatment: data from a prospective multicenter study performed within the French Centre d’Étude et de Conservation des Oeufs et du Sperme network

Sperm DNA Fragmentation Index and Hyaluronan Binding Ability in Men from Infertile Couples and Men with Testicular Germ Cell Tumor

Short‐term FSH treatment and sperm maturation: a prospective study in idiopathic infertile men

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