Specular microscopy to determine corneal endothelial cell morphology and morphometry in chinchillas (<i><scp>C</scp>hinchilla lanigera</i>) <i>in vivo</i>

Bercht, Bernardo Stefano; Albuquerque, Luciane de; Araújo, Ana Cristina Pacheco de; Pigatto, João Antônio Tadeu

doi:10.1111/vop.12236

Cited by 20 publications

(46 citation statements)

References 29 publications

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“…Using images obtained by specular microscopy, it is possible to examine the corneal endothelium and obtain data related to endothelial cell density and morphology. This technique has been used to quantify endothelial parameters in humans and other animal species including pigs (TAMAYO-ARANGO et al, 2009), rabbits (SAILSTAD & PEIFFER, 1981;OJEDA et al, 2001), dogs (GWIN et al, 1982;PIGATTO et al, 2006;RODRIGUES et al, 2006), horses (ANDREW et al, 2001;LEDBETTER & SCARLETT, 2009), llamas and alpacas (ANDREW et al, 2002), chinchillas (BERCHT et al, 2015), and cats (FRANZEN et al, 2010), among others. The high cost of specular microscopes and the difficulty in obtaining good images in injured endothelial areas are common challenges or barriers to the use of this technique (ANDREW et al, 2001;PIGATTO et al, 2005a;SAAD et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Morphology of endothelial cells from different regions of the equine cornea

et al. 2016

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“…The results observed in this study show that normal swine corneal endothelium consisted of a continuous monolayer with mainly hexagonal cells, but were also found cells with five sides and with seven sides. Previous studies conducted involving vertebrate animals have shown that there is predominance of cells with hexagonal shape and, in lower proportion, there is presence of five-and seven-sided cells (Collin & Collin, 1998, Pigatto et al 2005, Gum et al 2007, Tamayo-Arango et al 2009, Bercht et al 2015, Coyo, et al 2015.…”

Section: Discussionmentioning

confidence: 97%

“…The parameters evaluated by specular microscopy did not differ significantly between the right eye bulb and the left eye bulb from the same pig. It is well established that the density of endothelial cells from both healthy eyes of the same patient is similar (Pigatto et al 2005, Gum et al 2007, Tamayo-Arango et al 2009, Bercht et al 2015, Coyo, et al 2015. The examination of specular microscopy performed before starting of the experiment allowed to only be selected healthy eyes.…”

Section: Discussionmentioning

confidence: 99%

Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

et al. 2016

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ABSTRACT.-Terzariol M., Hünning P.S., Brambatti G., Albuquerque L., Neumann C. & Pigatto J.A. T. 2016 The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue ® ) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.INDEX TERMS: Eye, brilliant blue 0.05%, toxicity, corneal endothelium, swine.

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“…Due to the fact that there is no cellular retraction and it can be used in animals and living humans, specular microscopy is considered the gold standard technique for assessing the corneal endothelium in people and animals (MORITA, 1995;ABIB & BARRETO, 2001;PIGATTO et al, 2006;PIGATTO et al, 2008;FRANZEN et al, 2010;BERCHT et al, 2015;ALBUQUERQUE et al, 2015;COYO et al, 2016). Specular microscopy has also been employed in the evaluation of dogs undergoing cataract removal surgery (NAGATSUYU et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Specular microscopy is among the most reliable techniques for analysing the corneal endothelium (ABIB & BARRETO, 2001;NAGATSUYU et al, 2014). Normal endothelial parameters have already been established in some species using a specular microscope, including: humans, sheep, dogs, monkeys, horses, cats, and others (ABIB & BARRETO, 2001;ANDREW, S. et al 2001;PIGATTO et al, 2006;PIGATTO et al, 2008;FRANZEN et al, 2010;BERCHT et al, 2015;COYO et al, 2016;TERZARIOL et al, 2016). Furthermore, specular microscopy is beginning to be used as part of the ophthalmic examination before and after cataract removal procedures in dogs (NAGATSUYU et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

et al. 2017

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The goal of this study was to describe the normal values of corneal endothelial cell density and hexagonality in healthy rabbits’ eyes using contact specular microscopy. Both eyes of 18 rabbits, males and females, of different ages were evaluated. The eyes were divided into three groups of 12 each according to the age range of the animals: G1 (6 months old), G2 (12 months old), and G3 (48 months old). Parameters studied included mean endothelial cell density and hexagonality. For Group I, the mean cell density was 2307 cells per mm2. For GII, the mean cell density was 1895 cells per mm2. For G III, the mean cell density was 1818 cells per mm2. Cell density decreased significantly with aging (P<0.001) among the three groups. Pleomorphism in the rabbits of GI was 74.33±10.08. In the rabbits of GII it was 71.83±11.38. In the rabbits of GIII it was 64.02±28.80. Significant differences (P<0.001) were evident between GI and GIII and GII and GIII but not between Groups I and II. The results showed a decrease in number of cells and hexagonality with aging. Corneal endothelium of rabbits suffers changes due to advancing age both in terms of cell density and morphology.

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Specular microscopy to determine corneal endothelial cell morphology and morphometry in chinchillas (Chinchilla lanigera) in vivo

Abstract: The results suggested that chinchilla corneal endothelium undergoes age-related changes. Moreover, with advancing age, the mean cell area increased and cell density decreased.

Cited by 20 publications

References 29 publications

Morphology of endothelial cells from different regions of the equine cornea

Morphology of endothelial cells from different regions of the equine cornea

Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

Corneal endothelial cell density and morphology in rabbits’ eyes using contact specular microscopy

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