Spectroscopic study on nystatin conformational modifications generated by its interaction with the solvent

Lupan, L.; Bandula, Rodica; Vasilescu, Marilena; Bercu, C.

doi:10.1007/s0021663550409

Cited by 10 publications

(2 citation statements)

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“…Withdrawn samples were immediately filtered through Whatman filter paper and assayed spectrophotometrically for Nys and AmB content in triplicate by an UV/VIS spectrophotometer (Shimadzu 1800, Kyoto, Japan) at a maximum absorption wavelength, λ max , 305 and 416 nm, respectively [ 56 , 76 , 77 ], using a standard calibration curve.…”

Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

et al. 2021

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Novel biodegradable and biocompatible formulations of “old” but “gold” drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (~50 µm). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.

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Section: Methodsmentioning

confidence: 99%

Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

et al. 2021

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“…Several analytical methods in the literature describing the determination of miconazole nitrate [10][11][12][13][14] or nystatin [15][16][17][18][19] alone or in combination with other drugs have been reported. We have previously developed in our laboratory HPLC and chemometric methods for the simultaneous determination of MIC and NYS in their pharmaceutical formulations [20].…”

Section: Introductionmentioning

confidence: 99%

Comparative determination of miconazole, nystatin, hydrocortisone and neomycin by HPTLC/HPLC-DAD

Salama

Gomaa

2013

Eur. J. Chem.

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KEYWORDSTwo new validated methods for the quantification of miconazole (MIC), nystatin (NYS), hydrocortisone acetate (HCA) and neomycin (NS) by high performance thin layer chromatography (HPTLC)-densitometry and reverse-phase high-performance liquid chromatography procedure coupled with photodiode array detector (RP-HPLC-DAD) were developed and compared. HPTLC separations were performed using a mobile phase, ethylacetate:methanol:glacial acetic acid (60:40:0.4, v:v:v) for MIC, NYS, HCA and ethylacetate for NS. For HPLC-DAD analysis, using an isocratic elution system, separation of all compounds was achieved. Good resolution and quantization were achieved, accuracy and precision, as well as detection and quantitation limits of the two methods, were evaluated and compared. Excellent linearity was observed for all of the standard calibration curves, and the correlation coefficients were above 0.9997. HPTLC limits of quantitation were 15.37×10 -2 , 9.8×10 -2 , 13.32×10 -2 and 15.19×10 -2 µg/mL for MIC, NYS, HCA and NS respectively, whereas HPLC limits were 6.80×10 -2 , 6.56×10 -2 , 1×10 -1 and 10.16×10 -2 µg/band for MIC, NYS, HCA and NS respectively. In comparison with HPLC, HPTLC is less expensive and faster procedure, requiring 2-3 h to analyse 10-12 samples on a single plate. The developed methods were proved to be specific, robust and accurate for the determination of cited drugs in pharmaceutical preparations.

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Simultaneous determination of hydrocortisone, oxytetracycline and nystatin by liquid chromatography

Gallego

Arroyo

2002

Chromatographia

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SummaryA reverse phase high performance liquid chromatographic (HPLC) method to determine Hydrocortisone (HYD), Oxytetracycline (OXY) and Nystatin (NYS) has been developed. The separation was made on a LichrCART | C18 column using methanol-NaH2PO4/Na2HPO4 gradient elution and a diode-array detector measuring at the maximum absorption wavelength for each compound. Quantification limits ranging from 0.19 to 0.85 gg L 1. were obtained. The method was applied to synthetic mixtures and pharmaceutical formulations. Analyses were made from the stock solutions and by means of standard addition. Both methods showed similar results, and the claimed levels of pharmaceutical preparations were sown to be in agreementwith the results from our analytical method.

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Spectroscopic study on nystatin conformational modifications generated by its interaction with the solvent

Abstract: Conformational transformations of the tetraenic nystatin chromophore under the influence of solvents and UV irradiation have been studied by absorption, fluorescence and wide-line NMR spectroscopy.

Cited by 10 publications

References 4 publications

Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

Comparative determination of miconazole, nystatin, hydrocortisone and neomycin by HPTLC/HPLC-DAD

Simultaneous determination of hydrocortisone, oxytetracycline and nystatin by liquid chromatography

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