Comparative determination of miconazole, nystatin, hydrocortisone and neomycin by HPTLC/HPLC-DAD

Abstract

KEYWORDSTwo new validated methods for the quantification of miconazole (MIC), nystatin (NYS), hydrocortisone acetate (HCA) and neomycin (NS) by high performance thin layer chromatography (HPTLC)-densitometry and reverse-phase high-performance liquid chromatography procedure coupled with photodiode array detector (RP-HPLC-DAD) were developed and compared. HPTLC separations were performed using a mobile phase, ethylacetate:methanol:glacial acetic acid (60:40:0.4, v:v:v) for MIC, NYS, HCA and ethylacetate for NS. For HPLC-DAD analysis, using an isocratic elution system, separation of all compounds was achieved. Good resolution and quantization were achieved, accuracy and precision, as well as detection and quantitation limits of the two methods, were evaluated and compared. Excellent linearity was observed for all of the standard calibration curves, and the correlation coefficients were above 0.9997. HPTLC limits of quantitation were 15.37×10 -2 , 9.8×10 -2 , 13.32×10 -2 and 15.19×10 -2 µg/mL for MIC, NYS, HCA and NS respectively, whereas HPLC limits were 6.80×10 -2 , 6.56×10 -2 , 1×10 -1 and 10.16×10 -2 µg/band for MIC, NYS, HCA and NS respectively. In comparison with HPLC, HPTLC is less expensive and faster procedure, requiring 2-3 h to analyse 10-12 samples on a single plate. The developed methods were proved to be specific, robust and accurate for the determination of cited drugs in pharmaceutical preparations.

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“…It is marketed as injection, tablet, cream etc. Various analytical methods have been developed for the pharmaceutical analysis of Miconazole alone or in combination with other drugs using HPLC [12][13][14][15] , HPTLC [15][16][17] , GC 18 , Derivative spectroscopy 19 method of analysis etc.…”

Application of TLC-Spectrodensitometric and Chemometric Methods for Determination of Momenta® Cream: A Comparative Study Applied on Ternary Mixture

“…Miconazole Nitrate (MIC) an imidazole antifungal agent that is used topically and by intravenous infusion, miconazole nitrate is the nitrate salt form of miconazole, an antifungal synthetic derivative of imidazole and used in the treatment of candidal skin infections. A review of the literature showed that methods reported for the determination of (MIC) alone or in combinations were spectrophotometry [21][22][23][24][25][26][27][28], HPLC [29][30][31][32][33][34][35], HPTLC [31,[36][37][38], gas chromatography [39,40], and electrochemical methods [41]. Gentamicin (GM) is a mixture of isomeric aminoglycoside antibiotics (gentamicin C 1 , gentamicin C 1A , and gentamicin C 2 ) produced by Micromonospora purpurea or M. echinospora.…”

Development and validation of a spectroscopic method for the simultaneous analysis of miconazole nitrate and hydrocortisone acetate in pharmaceutical dosage form

“…There are numerous literature evidences to demonstrate the analysis of drugs from combination dosage forms. These emphasize either separation of individual components or simultaneous analysis by using different advanced analytical methods such as high pressure liquid chromatography (HPLC) [2][3][4] Gas chromatography [5][6][7] HPTLC [8,9], fluoromertric method [10], micellar electrokinetic chromatographic method [11], bioanalytical method [12], photochemical reaction [13] and voltametric methods [14]. Although these techniques are reported with improved sensitivity for the measurement of the minute quantities of medicines in a dosage forms, these techniques are expansive to install and requires a higher degree of operator skill.…”