Spectrophotometric Studies on the Reaction of Diaveridine with Some Sulfonphthalein Dyes Based on Ion-Pair/Ion-Associate Complexes Formation

Abstract

The ion-associate complexes of diaveridine were prepared in a solution and studied spectrophotometrically. The bulky counter anions such as sulfonphthalein acidic dyes, namely, bromocresol green (I), bromophenol blue (II), bromothymol blue (III), bromocresol purple (IV), bromocresol blue (V), o-cresol red (VI), p-cresol blue (VII), and m-cresol purple (VIII) were used for ion-associate complexes formation. The optimal characteristics for the color formation and the stoichiometry of the reaction were evaluated. Spectral characteristics and stability constants of the formed ion associates are discussed in terms of nature of donor and acceptor molecular structures. The molar absorptivities and association constants for the colored complexes were evaluated using the Benesi-Hildbrand equation. Conformity to Beer’s law enabled the assay of dosage form of the drug. The molar absorptivity, specific absorptivity, Sandell sensitivity, correlation coefficient, and detection and quantification limits were also calculated. The methods were validated in terms of accuracy, specificity, precision, and linearity. Also, spectrophotometric determination of the drug in pure and pharmaceutical preparations was tested.

Association of 5,5′-Dibromo-o-Cresolsulfonphthalein Anions with Dye Cations in Aqueous Solution

“…3,4 In particular, they are used in technologies for determining the acidity of natural waters [5][6][7] and soils, [8][9][10] and also form the basis of sensitive elements of fiber optic sensors or devices. [11][12][13][14][15] One of the most common areas of application is the use of bromine derivatives as analytical reagents, including the determination of components in biosamples and pharmaceuticals: antiviral, 16 antiseptic, 17,18 bactericidal, [19][20][21] antitumor, 22 antihypertensive, 23 antitussives 24,25 and others. [26][27][28][29] The effective use of 5,5′-dibromo-o-cresolsulfonphthalein (DBS) in the technologies of recent years is known.…”

“…42 It is noteworthy that many applications are based on the formation of ion pairs (associates) between the anionic form of sulfonphthalein and the analyte . 5,10,[17][18][19][20][21][22][23][24][25][26][27][28][29] In some cases, such associates can be extracted from the aqueous to organic phases. 17,22,27 Analysis of publications indicates the need for indepth study of cation-anionic interactions that cause the formation of associates, which include anions (HAn -, An 2-) of DBS.…”

Simultaneous Quantification of Diaveridine and Sulfadimidine by Derivative and Ratio Derivative UV Spectroscopy

“…Different techniques chromatography [11,12], electro analytical methods [13][14][15][16] and spectrofluorometric [17] were used. Spectrophotometry is the most important strategy [18,19], which has already been reported for the drug quantification as a single component [20][21][22][23][24][25][26][27][28]. On the basis of the overlap in the spectra of two compounds, they cannot be analyzed simultaneously by direct spectrophotometry.…”