The formation of associates between one- or two-charged anions of sulfonephthalein dyes – thymolsulfonephthalein, cresolsulfonephthalein – and cyanine cations (pinacyanol, quinaldine red) has been considered in aqueous solution. Equilibrium association constants have been determined using spectrophotometric data. By semiempirical methods the enthalpy of formation and the eventual structure of ions and associates have been established.
The search for biologically active compounds that regulate liver function in fibrosis is an urgent medical and biological problem. A working hypothesis was tested, according to which low molecular weight biologically active compounds from Pleurotus ostreatus and Sacharamirses cerevisiae are capable of exerting immunomodulatory and antitoxic effects after intoxication of the body with ions of heavy metals, in particular copper sulfate. Elimination of the toxic effect caused by copper sulfate can also ensure the normalization of liver function in various pathologies, in particular with liver fibrosis. When determining toxicity, a study was carried out on Wistar rats, and when studying the effect of low molecular weight biologically active compounds on liver function, clinical trials were carried out on volunteers. The activity of alanine aminotransferase, aspartate aminotransferase, actonitase and glutathione peroxidase, as well as the content of bilirubin and lipid hydroperoxides were determined. It was shown that preliminary administration of biologically active compounds to rats at a dose of 0.05 mL/100 g of body weight provided the formation in some animals (up to 80%) of resistance to the toxic effect of copper sulfate (dose 2.5 mg/100 g of body weight). Such stability is associated with a shift in the balance of “prooxidants-antioxidants” towards antioxidants. The data obtained in the clinic on volunteers with liver fibrosis and hepatitis also testify in favour of the membranotropic action of biologically active compounds. Biologically active compounds provided a decrease or complete restoration of the activity of transferases (ALT and AST) in the blood serum of these patients, with the exception of one patient out of 20 examined. Our experiment has shown the relationship between the elimination of toxicity to the action of copper sulfate and the normalization of liver function in patients. The results obtained indicate that it will be promising to use a complex of low molecular weight components from P. ostreatus and S. cerevisiae as an antidote and hepatoprotective agent.
The possibility of associates formation between complex ferrumcyanide anions [Fe(CN) 6 ] 3-, [Fe(CN) 6 ] 4and cationic polymethine dye pinacyanol in aqueous solution has been investigated. Energetic characteristics and the structures of associates were determined and the influence of ionic surfactants on the associates has been considered.
The results of applying microscopic methods of analysis of pig feces to assess the digestibility of feed and diagnose various diseases of the gastrointestinal tract have been presented in the article. The microscopic method allows you to identify detritus, undigested fi ber, raw fat, fragments of animal feed ingredients, starch in the feces, gives an idea of the presence of foreign impurities (due to perverted appetite, contamination of feed), helminths, their eggs and other intestinal parasites. A number of preparations have been prepared to identify feed nutrients and feed components of the diet, and a number of coloring reagents were also used: Lugol’s solution for recognizing starch and its cleavage products (amylodextrin and erythrodextrin), Saathof’s reagent for detecting fat, and Hecht’s reagent for diff erentiating fat elements. The researches have been carried out under various magnifi cations of the microscope. Diff erent levels of detritus and undigested elements of feed in animals under the same conditions of rearing and feeding can identify individual features of digestion. The microscopic method of analysis does not require expensive equipment, reagents, and allows you to get the results of assessing the digestibility much faster than the methods of classical “wet” chemistry. This research method can be used by nutritionists to correct diets when using exogenous feed enzymes: phytase, xylanase, gluconase, amylase, mannanase, lipase, protease, etc., and provide results for discussion about the presence or absence of matrix eff ects from their use. The results of feces microscopy can give then idea of the quality of feed ingredients used: the content of non-starchy polysaccharides and the level of grain viscosity, the quality of animal ingredients (meat-bone, feather, fi sh meal), the level of indigestible fi ber in sunfl ower and soybean meals.
Викладені матеріали з вивчення білковості та сиро-придатності молока, отриманого від корів трьохпорід: симентальської, червоно-рябої та чорно-рябої.До завдань досліджень входило визначення фракцій-ного (за масовою часткою казеїну та його фракцій,альбуміну та глобулінів) і амінокислотного складубілків (співвідношення незамінних і замінних аміноки-слот, їх скори, визначення лімітуючих амінокислотбілка молока кожної з порід), швидкості згортаннята якості згустку молока, одержаного під дієюсичужного ферменту. The article describes the materials of study of protein andcheese making quality of milk from cows of three breeds:Simmental, Red and White and Black and White. The tasks ofthe research was to determine the fractional composition (massfraction of casein and its fractions, albumin and globulin) andamino-acid composition of proteins (the ratio of essential andnonessential amino acids, their scores, the definition of limitingamino acids in the protein of milk of each breed), the rate ofcoagulation and quality of milk clot formed under the action ofrennet.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.