Simultaneous Quantification of Diaveridine and Sulfadimidine by Derivative and Ratio Derivative UV Spectroscopy

Abstract

Derivative spectrophotometry for two sulfa drugs (Diaveridine and sulfadimidine) was carried out for their simultaneous quantification in their binary mixtures. The optimum conditions were studied. The zero crossing wavelengths for Diaveridine (DV) in presence of sulfadimidine (SDM) were 235, 273, 242, 258, 244 nm, 238 and 289, 224 nm for 1st, 2nd, 3rd and 4th derivatives, respectively. The linear range for DV was 2.5-35.0 μg/mL and that for SDM was 1.0-25.0 μg/mL. LODs for DV

Second and Third Derivative Spectrophotometric Determination of 3,3′,5-triidothyronine and thyroxine in Human Serum

“…The applications of derivation enable the separation of hidden signals and use them for quantitative purposes, because derivative leads to sharper zeroorder bands and gives higher signals in the resulting spectra. The spectra characteristics, for example peak height and noise level based on the parameters choice (19,20).…”