Spectrophotometric Flow-Injection Analysis for Hypoxanthine Based on the Detection of Superoxide Anion

Sarker, Ashok K.; Ukeda, Hiroyuki; Kawana, Daisuke; Sawamura, Masayoshi

doi:10.2116/analsci.15.1141

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…[1][2][3][4] Various methods, including spectrophotometry, chromatography and so on have been reported on the determination of xanthine. [5][6][7][8] However, these methods are costly and laborious, as well as the time of sample treatment and the need for skilled operators, alternative systems must be developed for this particular application.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking

Liu

Wei

et al. 2012

Chin. J. Chem.

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Here in this paper, xanthine oxidase (XOD) was immobilized onto the chitosan (CHT) modified electrode by a simple way of cross-linking with glutaraldehyde (GTD) and 3-aminopropyltriethoxysilane (KH). The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 µmol/L with a low detection limit of 0.0215 μmol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxanthine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.

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Section: Introductionmentioning

confidence: 99%

Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking

Liu

Wei

et al. 2012

Chin. J. Chem.

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“…The applications of diaphorase coupled with tetrazolium salts, which produce highly colored formazan dyes upon NADH oxidation, have been used as chromogenic reagents for quantification of NADH as detection signal [15]. In this study, we explored the use of dual enzymes system GLDH/Diaphorase in combination with MTT for the determination of ammonium in aqueous solutions.…”

Section: Optimization Of An Optical Assay For Ammonium Employing the mentioning

confidence: 99%

An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment

Azmi¹,

Abdullah²,

Ahmad³

et al. 2012

AJAC

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A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of glutamate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 -70 µM (R 2 = 0.9955) with detection limit of 11 µM. A good agreement (R 2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.

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Examination of performance of glassy carbon paste electrode modified with gold nanoparticle and xanthine oxidase for xanthine and hypoxanthine detection

Çubukçu

Tımur

Anık

2007

Talanta

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Spectrophotometric Flow-Injection Analysis for Hypoxanthine Based on the Detection of Superoxide Anion

Cited by 7 publications

References 15 publications

Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking

Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking

An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment

Examination of performance of glassy carbon paste electrode modified with gold nanoparticle and xanthine oxidase for xanthine and hypoxanthine detection

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