Spectrophotometric Determination of Olanzapine by Its Oxidation with N-Bromosuccinimide and Cerium(IV)sulfate

Abstract

Three simple spectrophotometric methods have been described for the assay of olanzapine in its pure and pharmaceutical formulations. The direct method (A) is based on the drug oxidation with excess of N-bromosuccinimide in acidic medium and the two indirect methods (B and C) are based on the oxidation of the drug with excess of Nbromosuccinimide and cerium(IV)sulfate, followed by the reaction of the unconsumed oxidants with celestine blue. The calibration graphs were linear over the range 10 -120 μg mL -1 (method A), 0.5 -6.0 μg mL -1 (method B) and 0.6 -3.0 μg mL -1 (method C). After validation, the proposed methods were successfully applied to assay of olanzapine in its commercial tablets with mean percentage recoveries of 101.23 ± 0.10, 96 ± 0.10 and 94 ± 0.04%. The mechanism of olanzapine oxidation with N-bromosuccinimide was also proposed.

Sensitive Spectrophotometric Assay of Muscarinic Receptor Antagonist Tolterodine Tartrate in Bulk Drug and Pharmaceutical Formulations

“…CAS because of its high oxidation potential and excellent solution stability has been widely used as an effective analytical reagent in spectrophotometric methods for the determination of many pharmaceutical compounds [27][28][29][30][31]. The analytical reactions involved two steps; the first one was concerned with oxidation of TOL with a known excess of CAS in acidic medium at room temperature (25°C±2°C).…”

Determinação de olanzapina em formulações farmacêuticas por espectrofotometria: desenvolvimento e validação

“…Comparando-se o valor do desvio padrão relativo, verifica-se que os métodos utilizando etanol como solvente são mais precisos, porém todos os métodos propostos são similares em termos de precisão aos métodos espectrofotométricos que utilizam reações com a olanzapina 11 (precisão intradia DPR = 0,62% e precisão interdia DPR = 1,57%) descritos na literatura e mais precisos que os métodos por difração de raio-X 22 (precisão intradia DPR = 2,1% e precisão interdia DPR = 7,8%).…”

“…5,6 A olanzapina não é oficialmente registrada em nenhuma farmacopéia, 7 por isso torna-se necessário o desenvolvimento e a validação de um método analítico que seja capaz de quantificar o fármaco nas especialidades farmacêuticas comercializadas no mercado. Existem na literatura científica vários estudos visando a quantificação de olanzapina em amostras biológicas e formulações farmacêuticas, dentre os quais se destacam os métodos cromatográficos, 8,9 eletroforéticos, 10 eletroquími-cos, 10 espectrofotométricos, [10][11][12] injeção em fluxo, 1 cinéticos 13 e titulomé-tricos. 12 No entanto, a maioria destes métodos apresenta inconvenientes como, por exemplo, uso de equipamentos caros, 1,9,10 reagentes tóxicos 10,12 e procedimentos demasiadamente complexos 1,11 e demorados que os tornam pouco acessíveis e, em alguns casos, pouco viáveis.…”

Method development and Validation of RP-HPLC method for the Determination of Olanzapine in Bulk and Tablet Dosage form

“…It is chemically (2-methyl-4-(4-methyl-1-piperazinyl)-10H-thieno- [2,3b] [1,2] benzodiazepine. As per the literature review, olanzapine was estimated by few methods like spectrophotometric [1][2][3][4][5] liquid chromatography-mass spectrometry (MS) [6,7], high performance liquid chromatographic (HPLC) [8,9] capillary zone electrophoresis [10] and gas chromatography-MS [11]. Therefore, there is a need for a reliable, sensitive and rapid method for its analysis in bulk and pharmaceutical preparations.…”